中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE
2014年
9期
72-74
,共3页
高效液相色谱法%六味能消散%芦荟大黄素%大黄酸%大黄素%大黄酚%大黄素甲醚%含量测定
高效液相色譜法%六味能消散%蘆薈大黃素%大黃痠%大黃素%大黃酚%大黃素甲醚%含量測定
고효액상색보법%륙미능소산%호회대황소%대황산%대황소%대황분%대황소갑미%함량측정
HPLC%Liuwei Nengxiaosan%aloe emodin%rhein%emodin%chrysophanol%physcion%content determination
目的：建立测定藏药六味能消散中蒽醌类成分芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的含量测定方法。方法采用高效液相色谱法。色谱柱为Eclipse XDB C18柱(150 mm×4.6 mm,5μm),流动相为甲醇-0.1%磷酸溶液(88∶12),检测波长为254 nm,柱温为30℃,流速为1.0 mL/min。结果芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚分别在0.076～0.380μg(r＝0.9996)、0.068～0.340μg(r＝0.9998)、0.076～0.380μg(r＝0.9999)、0.070～0.349μg(r＝0.9999)、0.071～0.355μg(r＝0.9997)范围内与各自峰面积积分值呈良好线性关系,平均加样回收率分别为97.88%(RSD＝0.72%)、97.52%(RSD＝0.96%)、98.79%(RSD＝0.95%)、97.77%(RSD＝1.18%)、96.34%(RSD＝2.00%)。结论本方法简便、准确、重复性好,可用于藏药六味能消散的质量控制。
目的：建立測定藏藥六味能消散中蒽醌類成分蘆薈大黃素、大黃痠、大黃素、大黃酚和大黃素甲醚的含量測定方法。方法採用高效液相色譜法。色譜柱為Eclipse XDB C18柱(150 mm×4.6 mm,5μm),流動相為甲醇-0.1%燐痠溶液(88∶12),檢測波長為254 nm,柱溫為30℃,流速為1.0 mL/min。結果蘆薈大黃素、大黃痠、大黃素、大黃酚和大黃素甲醚分彆在0.076～0.380μg(r＝0.9996)、0.068～0.340μg(r＝0.9998)、0.076～0.380μg(r＝0.9999)、0.070～0.349μg(r＝0.9999)、0.071～0.355μg(r＝0.9997)範圍內與各自峰麵積積分值呈良好線性關繫,平均加樣迴收率分彆為97.88%(RSD＝0.72%)、97.52%(RSD＝0.96%)、98.79%(RSD＝0.95%)、97.77%(RSD＝1.18%)、96.34%(RSD＝2.00%)。結論本方法簡便、準確、重複性好,可用于藏藥六味能消散的質量控製。
목적：건립측정장약륙미능소산중은곤류성분호회대황소、대황산、대황소、대황분화대황소갑미적함량측정방법。방법채용고효액상색보법。색보주위Eclipse XDB C18주(150 mm×4.6 mm,5μm),류동상위갑순-0.1%린산용액(88∶12),검측파장위254 nm,주온위30℃,류속위1.0 mL/min。결과호회대황소、대황산、대황소、대황분화대황소갑미분별재0.076～0.380μg(r＝0.9996)、0.068～0.340μg(r＝0.9998)、0.076～0.380μg(r＝0.9999)、0.070～0.349μg(r＝0.9999)、0.071～0.355μg(r＝0.9997)범위내여각자봉면적적분치정량호선성관계,평균가양회수솔분별위97.88%(RSD＝0.72%)、97.52%(RSD＝0.96%)、98.79%(RSD＝0.95%)、97.77%(RSD＝1.18%)、96.34%(RSD＝2.00%)。결론본방법간편、준학、중복성호,가용우장약륙미능소산적질량공제。
Objective To set up a method for determining the content of anthraquinone in Liuwei Nengxiaosan.Methods The chromatogram column was Eclipse XDB C18 (150 mm×4.6 mm, 5μm), the mobile phase composition was methanol-0.1% orthphosphoric acid (88∶12), the detection wavelength was at 254 nm, the column temperature was 30℃, the flow rate was 1.0 mL/min. Results The linear range of aloe emodin, rhein, emodin, chrysophanol and physcion was 0.076-0.380μg (r=0.999 6), 0.068-0.340μg (r=0.999 8), 0.076-0.380μg (r=0.999 9), 0.070-0.349μg (r=0.999 9), 0.071-0.355μg (r=0.999 7), respectively. The average recovery rate respectively was 97.88% (RSD=0.72%), 97.52% (RSD=0.96%), 98.79% (RSD=0.95%), 97.77% (RSD=1.18%), 96.34% (RSD=2.00%).Conclusion The method is highly effective, quick and sensitive, suitable for quality control of Tibet traditional medicine Liuwei Nengxiaosan.
