CAJ | 학술논문

目的：观察双黄连注射液的类过敏反应，测定致敏动物血清组胺含量，探讨其与类过敏反应的相关性。方法将豚鼠随机分组，选用双黄连注射液按不同注射速度、不同剂量进行给药，观察豚鼠的类过敏反应，同时用ELISA进行血清免疫球蛋白E(IgE)、血清组胺含量检测。结果不同剂量、不同注射速度下的双黄连注射液均能引起豚鼠的类过敏症状；不同剂量、不同注射速度下的双黄连注射液组IgE水平与生理盐水组无明显差异；不同剂量、不同注射速度下的双黄连注射液组血清组胺水平明显升高，且呈剂量、速度相关性。结论双黄连注射液能引起豚鼠产生类过敏反应，豚鼠血清组胺含量的变化与双黄连注射液的给予剂量、速度有相关性。
목적：관찰쌍황련주사액적류과민반응，측정치민동물혈청조알함량，탐토기여류과민반응적상관성。방법장돈서수궤분조，선용쌍황련주사액안불동주사속도、불동제량진행급약，관찰돈서적류과민반응，동시용ELISA진행혈청면역구단백E(IgE)、혈청조알함량검측。결과불동제량、불동주사속도하적쌍황련주사액균능인기돈서적류과민증상；불동제량、불동주사속도하적쌍황련주사액조IgE수평여생리염수조무명현차이；불동제량、불동주사속도하적쌍황련주사액조혈청조알수평명현승고，차정제량、속도상관성。결론쌍황련주사액능인기돈서산생류과민반응，돈서혈청조알함량적변화여쌍황련주사액적급여제량、속도유상관성。
Objective To observe the anaphylactoid reactions of Shuanghuanglian injection, determine the serum histamine level of sensitized animal, and to investigate its correlation with anaphylactoid reactions.Methods The guinea pigs were randomly selected to observe the anaphylactoid reactions using Shuanghuanglian injections in the different injection speed or different doses, and the serum histamine and the serum IgE of sensitized animals were determined by ELISA. Results The anaphylactoid reactions were induced by Shuanghuanglian injections in the different conditions of injection speed and doses in guinea pigs. There were no obvious difference about the serum IgE level between Shuanghuanglian group and saline group were injected in injection speed and doses. The serum histamine level was significantly increased in Shuanghuanglian group both different conditions of injection speed and doses, and it related to doses and speed. Conclusion Shuanghuanglian injection can cause anaphylactoid reactions in guinea pigs, and the changes of serum histamine level are related to injection speed and doses of Shuanghuanglian injection.