CAJ | 학술논문

目的探讨miR-17-92在难治性急性淋巴细胞白血病(RALL)患者外周血淋巴细胞中的表达情况.方法用淋巴细胞分离液提取21例经2个疗程以上标准方案化疗未获缓解的RALL患者和15例体内敏感急性淋巴细胞白血病(ALL)患者外周血淋巴细胞,应用MTT法检测该36例白血病患者淋巴细胞对多柔比星和顺铂的敏感性,从体外实验结果与临床疗效相符患者的淋巴细胞中提取总RNA,应用实时荧光定量PCR法检测样品中miR-17-92的表达情况.结果 MTT法细胞敏感性实验表明,18例RALL患者淋巴瘤细胞抑制率(IR)＜30％,为不敏感或耐药,14例体内敏感ALL患者IR＞30％,为中度敏感或敏感；将此32例患者纳入本次研究对象.实时荧光定量PCR检测显示,与对化疗药物敏感ALL患者组相比,RALL患者淋巴细胞中miR-17-92相对表达量升高(7.12±1.03比3.21±0.63,P＜0.05).结论 miR-17-92在RALL患者淋巴细胞中的表达水平相对较高,这可能与RALL多药耐药的发生、发展相关.
목적 탐토miR-17-92재난치성급성림파세포백혈병(RALL)환자외주혈림파세포중적표체정황.방법 용림파세포분리액제취21례경2개료정이상표준방안화료미획완해적RALL환자화15례체내민감급성림파세포백혈병(ALL)환자외주혈림파세포,응용MTT법검측해36례백혈병환자림파세포대다유비성화순박적민감성,종체외실험결과여림상료효상부환자적림파세포중제취총RNA,응용실시형광정량PCR법검측양품중miR-17-92적표체정황.결과 MTT법세포민감성실험표명,18례RALL환자림파류세포억제솔(IR)＜30％,위불민감혹내약,14례체내민감ALL환자IR＞30％,위중도민감혹민감；장차32례환자납입본차연구대상.실시형광정량PCR검측현시,여대화료약물민감ALL환자조상비,RALL환자림파세포중miR-17-92상대표체량승고(7.12±1.03비3.21±0.63,P＜0.05).결론 miR-17-92재RALL환자림파세포중적표체수평상대교고,저가능여RALL다약내약적발생、발전상관.
Objective To investigate the expression levels of miR-17-92 in peripheral blood lymphocytes of patients with refractory acute lymphocytic leukemia(RALL).Methods Peripheral blood lymphocytes of 21 RALL patients and 15 chemotherapy-sensitive ALL patients in vivo were isolated respectively.MTT assay was carried out to evaluate the sensitivity of lymphocytes to adriamycin (ADM)and cisplatin(DDP)in patients.Total RNA was extracted from lymphocytes of blood samples from 32 patients.The real-time PCR method was performed to detect the expression levels of miR-17-92.Results The MTT assay results showed that the inhibition rates(IR)in 18 RALL patients were less than 30％,which were sensitive or drug resistant,and the IR in 14 chemotherapy-sensitive ALL patients were higher than 30％,which were chemotherapy-sensitive.The relative expression levels of miR-17-92 in lymphocytes of RALL patients and chemotherapy-sensitive ALL patients were 7.12±1.03 and 3.21±0.63 respectively,which showed that compared with the chemotherapy-sensitive ALL patients,the relative expression of miR-17-92 in lymphocytes of RALL patients was significantly increased(P＜0.05).Conclusions The expression level of miR-17-92 in lymphocytes of RALL patients is up-regulated,miR-17-92 may have relationship with the occurrence and development of multi-drug resistance in RALL.