CAJ | 학술논문

为了建立鱼粉中组胺药物残留量测定的柱前衍生-高效液相色谱法，将样品匀浆后经高氯酸水溶液超声提取，提取液经丹酰氯衍生后，采用紫外检测器在254 nm处对组胺酮进行检测。结果表明，组胺分离效果好，检出限（LOD）为25.0 mg/kg，定量限（LOQ）为50.0μg/kg，相对标准偏差为2.11%～4.76%（n=6），加标回收率达到79.5%～90.1%。该方法具有比较高的重现性和选择性，适用于鱼粉中组胺的残留测定。
위료건립어분중조알약물잔류량측정적주전연생-고효액상색보법，장양품균장후경고록산수용액초성제취，제취액경단선록연생후，채용자외검측기재254 nm처대조알동진행검측。결과표명，조알분리효과호，검출한（LOD）위25.0 mg/kg，정량한（LOQ）위50.0μg/kg，상대표준편차위2.11%～4.76%（n=6），가표회수솔체도79.5%～90.1%。해방법구유비교고적중현성화선택성，괄용우어분중조알적잔류측정。
Established an analytical method for the determination of histamine in fish meal by high performance liquid chromatography with pre-column derivatization. The homogenated sample were ultrasonically extracted with perchloric acid aqueous solution, derivatized with dansylchloride,detected with a U V detectorat254 nmforhistamine.The resultshowed thatthe histamine can be separated efficiently.The detection limits of this method were 25.0μg/kg, and the limits of quantification were 50.0μg/kg, The relative standard deviations ranging from2.11% ~4.76% (n=6)and there covery rates were from 79.5% ~90.1% .The method proposed by this paper possesses higher prodicibility and selectivity,Itis promising in realapplications ofdetermining the histamine in fish meal.