湖南农业科学
湖南農業科學
호남농업과학
HUNAN AGRICULTURAL SCIENCES
2014年
14期
33-35,37
,共4页
松墨天牛%杀虫剂%肌肉蛋白基因%RT-qPCR
鬆墨天牛%殺蟲劑%肌肉蛋白基因%RT-qPCR
송묵천우%살충제%기육단백기인%RT-qPCR
Monochamusalternatus Hope%pesticide%muscle protein gene%realtime quantitative PCR
松墨天牛（Monochamusalternatus Hope）是松树的重要蛀干害虫，也是松材线虫（Bursaphelenchusxylophilus）的主要传播媒介。试验从松墨天牛文库中分离得到肌肉LIM蛋白基因的部分cDNA序列，长度为871 bp，命名为MaLIM（GenBank：KJ872589）；与赤拟谷盗（Tribolium castaneum）和家蚕（Bombyxmori）氨基酸相似性分别为77%和75%。用RT-qPCR测定了11种杀虫剂对MaLIM相对表达量的影响，结果表明，杀虫剂处理后MaLIM基因的表达量均有所下降，仅为对照的0.03~0.53。
鬆墨天牛（Monochamusalternatus Hope）是鬆樹的重要蛀榦害蟲，也是鬆材線蟲（Bursaphelenchusxylophilus）的主要傳播媒介。試驗從鬆墨天牛文庫中分離得到肌肉LIM蛋白基因的部分cDNA序列，長度為871 bp，命名為MaLIM（GenBank：KJ872589）；與赤擬穀盜（Tribolium castaneum）和傢蠶（Bombyxmori）氨基痠相似性分彆為77%和75%。用RT-qPCR測定瞭11種殺蟲劑對MaLIM相對錶達量的影響，結果錶明，殺蟲劑處理後MaLIM基因的錶達量均有所下降，僅為對照的0.03~0.53。
송묵천우（Monochamusalternatus Hope）시송수적중요주간해충，야시송재선충（Bursaphelenchusxylophilus）적주요전파매개。시험종송묵천우문고중분리득도기육LIM단백기인적부분cDNA서렬，장도위871 bp，명명위MaLIM（GenBank：KJ872589）；여적의곡도（Tribolium castaneum）화가잠（Bombyxmori）안기산상사성분별위77%화75%。용RT-qPCR측정료11충살충제대MaLIM상대표체량적영향，결과표명，살충제처리후MaLIM기인적표체량균유소하강，부위대조적0.03~0.53。
Monochamus alternatus H ope is one of the important pests of pine stemborers,and also is vector of pine wood nematode (Bursaphelenchusxylophilus).Inthisstudy,apartialcDNA sequenceofmuscleLIMproteingene with870bpinlengthwasisolatedfromM.alternatuslibrary and named MaLIM(G enB ank:K J872589) thathas 77% and 75% amino acid similarity with the muscle L IMprotein of Triboliumcastaneumand Bombyx mori respectively. T he relative expression of MaLIMin response to 11 kinds of insecticides was determined by R T-qPC R ,the results showed thatthe MaLIMwas down regulated and the expression levelwas changed from0.03 times to 0.53 times of the control.
