中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2014年
15期
1-3
,共3页
李东燕%韩红波%卢清军%李洪林
李東燕%韓紅波%盧清軍%李洪林
리동연%한홍파%로청군%리홍림
二甲双胍%SMMC-7721%表皮生长因子%细胞凋亡
二甲雙胍%SMMC-7721%錶皮生長因子%細胞凋亡
이갑쌍고%SMMC-7721%표피생장인자%세포조망
Metformin%SMMC-7721%Epidermal growth factor%Apoptosis
目的：观察二甲双胍对人肝癌 SMMC-7721细胞表皮生长因子（EGF）及其受体（EGFR）的影响。方法用不同浓度二甲双胍作用于体外培养的人肝癌 SMMC-7721细胞后，MTT 法检测药物对肝癌细胞增殖的抑制情况；流式细胞仪及 Hoechst33342染色荧光显微镜观察细胞凋亡；免疫印迹法检测 EGF 及 EGFR 的表达。结果二甲双胍能明显抑制肝癌细胞的生长增殖，且呈剂量依赖性；流式细胞仪检测显示：随着二甲双胍作用浓度的增加，细胞凋亡率逐渐增加，二甲双胍实验组细胞凋亡率分别为（10.76±0.96）%、（20.77±1.16）%，Hoechst33342染色可见凋亡细胞皱缩，核染色质浓缩，核碎裂，亦可见典型的凋亡小体；western-blot 显示：随着二甲双胍作用浓度的增大，EGF 及 EGFR 表达逐渐下调。结论二甲双胍可抑制人肝癌细胞 SMMC-7721的增殖，并促进其凋亡，其机制可能与下调细胞内 EGF 及 EGFR 的表达有关。
目的：觀察二甲雙胍對人肝癌 SMMC-7721細胞錶皮生長因子（EGF）及其受體（EGFR）的影響。方法用不同濃度二甲雙胍作用于體外培養的人肝癌 SMMC-7721細胞後，MTT 法檢測藥物對肝癌細胞增殖的抑製情況；流式細胞儀及 Hoechst33342染色熒光顯微鏡觀察細胞凋亡；免疫印跡法檢測 EGF 及 EGFR 的錶達。結果二甲雙胍能明顯抑製肝癌細胞的生長增殖，且呈劑量依賴性；流式細胞儀檢測顯示：隨著二甲雙胍作用濃度的增加，細胞凋亡率逐漸增加，二甲雙胍實驗組細胞凋亡率分彆為（10.76±0.96）%、（20.77±1.16）%，Hoechst33342染色可見凋亡細胞皺縮，覈染色質濃縮，覈碎裂，亦可見典型的凋亡小體；western-blot 顯示：隨著二甲雙胍作用濃度的增大，EGF 及 EGFR 錶達逐漸下調。結論二甲雙胍可抑製人肝癌細胞 SMMC-7721的增殖，併促進其凋亡，其機製可能與下調細胞內 EGF 及 EGFR 的錶達有關。
목적：관찰이갑쌍고대인간암 SMMC-7721세포표피생장인자（EGF）급기수체（EGFR）적영향。방법용불동농도이갑쌍고작용우체외배양적인간암 SMMC-7721세포후，MTT 법검측약물대간암세포증식적억제정황；류식세포의급 Hoechst33342염색형광현미경관찰세포조망；면역인적법검측 EGF 급 EGFR 적표체。결과이갑쌍고능명현억제간암세포적생장증식，차정제량의뢰성；류식세포의검측현시：수착이갑쌍고작용농도적증가，세포조망솔축점증가，이갑쌍고실험조세포조망솔분별위（10.76±0.96）%、（20.77±1.16）%，Hoechst33342염색가견조망세포추축，핵염색질농축，핵쇄렬，역가견전형적조망소체；western-blot 현시：수착이갑쌍고작용농도적증대，EGF 급 EGFR 표체축점하조。결론이갑쌍고가억제인간암세포 SMMC-7721적증식，병촉진기조망，기궤제가능여하조세포내 EGF 급 EGFR 적표체유관。
Objective To observe the effect of metformin on the expression of EGF and EGFR in human liver cancer cell line SMMC-7721. Methods In vitro,the SMMC-7721 cells were treated with different concentrations of metformin,the proliferation of the cells was detected by MTT assay,and the apoptosis of the cells was measured by cytochemical staining with Hoechst33342 and flow cytometry. The expression of EGF and EGFR were investigated by western blot. Results Metformin inhibited the growth of SMMC-7721 cells obviously in a dose-dependent manner. FCM analysis showed that when SMMC-7721 cells were treated with metformin,the apoptosis rate for 48 h was(10. 76 ± 0. 96)% ,(20. 77 ± 1. 16)% ,respectively. Cell apoptosis with cell shrinkage,nuclear chromatin concentration and fragmen-tation as well as the formation of apoptotic bodies were observed by cytochemical staining when treated with metformin. The western-blot showed that the expressions of EGF and EGFR were down regulated while the concentration of metformin was increasing. Conclusions Metformin can inhibit cell prolifera-tion and induce apoptosis in liver cancer cell line SMMC-7721,which may be related to the down-regula-tion of EGF and EGFR protein expression.
