中国饲料
中國飼料
중국사료
CHINA FEED
2014年
16期
28-32
,共5页
张谦%贾佳%林智%郭宏涛%王剑英
張謙%賈佳%林智%郭宏濤%王劍英
장겸%가가%림지%곽굉도%왕검영
脂肪酶%黑曲霉%紫外线诱变%亚硝基胍诱变%复合诱变%高产菌株
脂肪酶%黑麯黴%紫外線誘變%亞硝基胍誘變%複閤誘變%高產菌株
지방매%흑곡매%자외선유변%아초기고유변%복합유변%고산균주
lipase%Aspergillus niger%ultraviolet mutation%nitrosoguanidine mutation%compound mutation%high-yielding strain
本研究以饲料用脂肪酶产生菌---黑曲霉（Aspergillus niger）G55为出发菌株，通过紫外线（UV）和亚硝基胍（NTG）诱变，结合自然分离纯化，选育出了遗传稳定的高产菌株uan370（s）和uan783（s），其产酶量分别为2410 U/mL和2486 U/mL，相比出发菌株G55提高32.1%和36.3%。通过斜面连续传5代，证明两菌株的遗传稳定性良好。
本研究以飼料用脂肪酶產生菌---黑麯黴（Aspergillus niger）G55為齣髮菌株，通過紫外線（UV）和亞硝基胍（NTG）誘變，結閤自然分離純化，選育齣瞭遺傳穩定的高產菌株uan370（s）和uan783（s），其產酶量分彆為2410 U/mL和2486 U/mL，相比齣髮菌株G55提高32.1%和36.3%。通過斜麵連續傳5代，證明兩菌株的遺傳穩定性良好。
본연구이사료용지방매산생균---흑곡매（Aspergillus niger）G55위출발균주，통과자외선（UV）화아초기고（NTG）유변，결합자연분리순화，선육출료유전은정적고산균주uan370（s）화uan783（s），기산매량분별위2410 U/mL화2486 U/mL，상비출발균주G55제고32.1%화36.3%。통과사면련속전5대，증명량균주적유전은정성량호。
This study was used to industrial produce lipase strains (Aspergillus niger G55).The strain as the starting strain was multated by ultraviolet (UV) and nitrosoguanidine (NTG),combined with the natural process of separation and purification.Breeding high-yielding strains with genetically stable uan 370 (s) and uan 783 (s),lipase activity were 2410 U/mL and 2486 U/mL.Compared with the parent strain G55,the activity increased by 32.1% and 36.3%.Two strains have been proved a good genetic stability by slant culture of five generations.
