CAJ | 학술논문

目的：了解临床分离的多耐药铜绿假单胞菌携带抗药基因qacE△1-sul 1（消毒剂-磺胺复合基因）情况及对常用消毒剂抗性水平。方法采用聚合酶链反应（PCR）法和悬液定量杀菌试验分别检测临床分离的多耐药铜绿假单胞菌qacE△1-sul 1基因和对三氯异氰尿酸、聚维酮碘、戊二醛的抗性（杀灭率）。结果临床分离的10株多耐药铜绿假单胞菌中6株qacE△1-sul 1基因阳性，4株阴性。浓度为125 mg/L的三氯异氰尿酸溶液作用1 min的杀灭率为99．63％～100．00％；浓度为250 mg/L 的三氯异氰尿酸溶液作用1 min 的杀灭率为99．81％～100．00％；浓度为125、250 mg/L的三氯异氰尿酸溶液作用3、5 min的杀灭率及浓度为500 mg/L时作用1、3、5 min的杀灭率均为100．00％。有效碘浓度为125 mg/L的聚维酮碘溶液作用0．5 min的杀灭率为99．91％～100．00％，该浓度作用1、3 min的杀灭率和浓度分别为250、500 mg/L时作用0．5、1、3 min的杀灭率均达100．00％。浓度为250 mg/L的戊二醛溶液作用1 min的杀灭率为65．46％～99．75％；浓度为500 mg/L的戊二醛溶液作用1 min的杀灭率为99．84％～100．00％，这两种浓度的戊二醛溶液作用3、5 min的杀灭率均达100．00％。结论多耐药铜绿假单胞菌抗药基因qacE△1-sul 1携带率较高，本研究显示消毒剂正确运用对多耐药铜绿假单胞菌能起到较好的杀灭效果。
목적：료해림상분리적다내약동록가단포균휴대항약기인qacE△1-sul 1（소독제-광알복합기인）정황급대상용소독제항성수평。방법채용취합매련반응（PCR）법화현액정량살균시험분별검측림상분리적다내약동록가단포균qacE△1-sul 1기인화대삼록이청뇨산、취유동전、무이철적항성（살멸솔）。결과림상분리적10주다내약동록가단포균중6주qacE△1-sul 1기인양성，4주음성。농도위125 mg/L적삼록이청뇨산용액작용1 min적살멸솔위99．63％～100．00％；농도위250 mg/L 적삼록이청뇨산용액작용1 min 적살멸솔위99．81％～100．00％；농도위125、250 mg/L적삼록이청뇨산용액작용3、5 min적살멸솔급농도위500 mg/L시작용1、3、5 min적살멸솔균위100．00％。유효전농도위125 mg/L적취유동전용액작용0．5 min적살멸솔위99．91％～100．00％，해농도작용1、3 min적살멸솔화농도분별위250、500 mg/L시작용0．5、1、3 min적살멸솔균체100．00％。농도위250 mg/L적무이철용액작용1 min적살멸솔위65．46％～99．75％；농도위500 mg/L적무이철용액작용1 min적살멸솔위99．84％～100．00％，저량충농도적무이철용액작용3、5 min적살멸솔균체100．00％。결론다내약동록가단포균항약기인qacE△1-sul 1휴대솔교고，본연구현시소독제정학운용대다내약동록가단포균능기도교호적살멸효과。
Objective To get a general view about drug-resistant gene qacE△1-sul 1(disinfectant-sulfanila-mide compound gene) carried by multidrug resistance pseudomonas aeruginosa collected from clinic ,and the resistant level against common disinfectants .Methods PCR(polymerase chain reaction) and suspension quantitative germicidal test were selected to study resistance (killing rate) of gene qacE △1-sul 1 carried by multidrug resistance pseudo-monas aeruginosa to trichloroisocyanuric acid ,iodophor ,and glutaraldehyde .Results 6 of 10 strains of multidrug re-sistance pseudomonas aeruginosa collected from clinic were qacE △1-sul 1 positive strains .The others were negative . The killing rate of 125 mg/L trichloroisocyanuric acid affected 1 minute was 99 .63% -100 .00% .The killing rate of 250 mg/L trichloroisocyanuric acid affected 1 minute was 99 .81% -100 .00% .The killing rate of the two solutions above affected 3 minutes and 5 minutes and the solution of 500 mg/L affected 1 ,3 ,5 minutes were all 100% .The kill-ing rate of solutions with effective iodine content of 125 mg/L affected 0 .5 minute was 99 .91% -100 .00% .The so-lution above affected 1 minute and 3minute along with the 250 mg/L and 500 mg/L solutions affected 0 .5 ,1 ,3 mi-nutes ,the killing rate were all 100% .The killing rate of 250 mg/L glutaraldehyde affected 1 minute was 65 .46% -99 .75% ,that of 500 mg/L solution affected 1minute was 99 .84% -100 .00% .Both of the solutions above affected 3 minuted and 5 minutes were 100% effective .Conclusion There′s a high carrying rate of gene qacE△1-sul 1 by mul-tidrug resistance pseudomonas aeruginosa .The research shows the proper use of the disinfectants can receive a good result of killing multidrug resistance pseudomonas aeruginosa .