CAJ | 학술논문

目的：探讨两种质谱仪 Biotyper MS 和 Vitek-MS 系统在临床常规分离细菌鉴定中的应用价值。方法收集沈阳军区总医院2012年3月至2013年1月分离自血液、尿液、脑脊液、分泌物和痰液等标本的临床常见菌细菌149株（包括14个菌属和30个菌种）。同时采用两种 MALDI-TOF-MS 系统对上述菌株进行鉴定，结果与 Vitek2 compact 常规生化鉴定进行比较，对3种方法检测结果不一致菌株用16S rRNA 测序进行确认。结果在149株常见细菌中，Bruker Biotype 属和种的正确鉴定率分别为98％和96％，Vitek-MS 属和种的正确鉴定率分别为97％和95％。两者均无错误鉴定菌株。结论对该组常见细菌，两种质谱仪鉴定结果比较差异无统计学意义（P ＞0．05），均呈现出优异的鉴定水平，适合用于临床微生物的常规鉴定。
목적：탐토량충질보의 Biotyper MS 화 Vitek-MS 계통재림상상규분리세균감정중적응용개치。방법수집침양군구총의원2012년3월지2013년1월분리자혈액、뇨액、뇌척액、분비물화담액등표본적림상상견균세균149주（포괄14개균속화30개균충）。동시채용량충 MALDI-TOF-MS 계통대상술균주진행감정，결과여 Vitek2 compact 상규생화감정진행비교，대3충방법검측결과불일치균주용16S rRNA 측서진행학인。결과재149주상견세균중，Bruker Biotype 속화충적정학감정솔분별위98％화96％，Vitek-MS 속화충적정학감정솔분별위97％화95％。량자균무착오감정균주。결론대해조상견세균，량충질보의감정결과비교차이무통계학의의（P ＞0．05），균정현출우이적감정수평，괄합용우림상미생물적상규감정。
Objective To evaluated the application value of two kinds of mass spectrometer(MS)and Vitek MS system in the i-dentification of routinely isolated bacteria in clinic.Methods 149 strains of common bacteria(including 14 genera and 30 species)i-solated from blood,urine,cerebral spinal fluid,secretion and sputum samples in our hospital from March 2012 to January 2013 were collected and simultaneously identified by 2 kinds of matrix-assisted laser desorption ionization-time of flight mass spectrometer (MALD-TOF-MS).The identification results were compared with those identified by the conventional biochemical identification (Vitek2 compact).The strains with the inconsistent results identified by 3 kinds of method were confirmed by 16S rDNA gene se-quencing.Results Among 149 common bacteria,the correct identification rates of genus and species by the Bruker Biotyper MS were 98% and 96% respectively and which by the Vitek MS system were 97% and 95% respectively.There were no misidentified bacterial strains by these two kinds of MS.Conclusion No statistical difference in the identification results was observed between these two kinds of MS system(P >0.05).Both exhibit excellent identification level and are suitable for the routine laboratory iden-tification of clinical microorganism.