CAJ | 학술논문

目的：研究 ICU 患者肠道定植菌与感染菌基因的同源性，为制定控制多重耐菌医院感染措施提供流行病学和分子生物学依据。方法对 ICU 患者肛拭子分离出的多重耐药定植菌与同一患者临床标本分离出的相同多重耐药菌进行配对，采用Diversilab 自动化重复序列-PCR 分型系统，分析肠道多重耐药定植菌与感染菌基因的同源性。结果选取4对多重耐药定植菌与患者入院时临床标本分离出的相同多重耐药菌进行同源性检测，2对有同源性、2对无同源性；选取4对多重耐药定植菌与患者医院感染临床标本分离出的相同多重耐药菌进行同源性检测，4对均有同源性。结论 ICU 患者多重耐药定植菌与感染菌基因具有同源性，多重耐药定植菌可以引起医院感染的发生，临床上应加强多重耐药定植菌患者的管理。
목적：연구 ICU 환자장도정식균여감염균기인적동원성，위제정공제다중내균의원감염조시제공류행병학화분자생물학의거。방법대 ICU 환자항식자분리출적다중내약정식균여동일환자림상표본분리출적상동다중내약균진행배대，채용Diversilab 자동화중복서렬-PCR 분형계통，분석장도다중내약정식균여감염균기인적동원성。결과선취4대다중내약정식균여환자입원시림상표본분리출적상동다중내약균진행동원성검측，2대유동원성、2대무동원성；선취4대다중내약정식균여환자의원감염림상표본분리출적상동다중내약균진행동원성검측，4대균유동원성。결론 ICU 환자다중내약정식균여감염균기인구유동원성，다중내약정식균가이인기의원감염적발생，림상상응가강다중내약정식균환자적관리。
Objective To study the gene homology of intestinal colonized and infectious bacteria in ICU patients to provide the epidemiological and molecular biological basis for formulating the control measures of multi resistant bacterial hospital infection. Methods The multi-drug resistant colonized bacteria isolated from the anal swabs and the same multi-drug resistant bacteria isola-ted from the clinical samples in the same patients were matched.The Diversilab automatic repetitive extragenic palindromic(REP)-PCR typing system was adopted to analyze the gene homology of multi-drug resistant colonized bacteria and infectious bacteria in the intestinal tract.Results 4 pairs of multi-drug resistant colonized bacteria and the same multi-drug resistant bacteria isolated from the clinical samples on admission in the same patients were selected and performed the homology detection,2 pairs had the ho-mology and 2 pairs had no homology;4 pairs of multi-drug resistant colonized bacteria and the same multi-drug resistant bacteria isolated from the clinical samples in the patients with hospital infection were performed the homology detection,4 pairs all showed the homology.Conclusion The multi-drug resistant colonized bacteria and the infectious bacteria in ICU patients have the homolo-gy.The multi-drug resistant colonized bacteria can cause the occurrence of hospital infection,so their management should be strengthened in clinic.