中国实用神经疾病杂志
中國實用神經疾病雜誌
중국실용신경질병잡지
CHINESE JOURNAL OF PRACTICAL NERVOUS DISEASES
2014年
18期
4-7
,共4页
何斐%李焕丽%陈强%郭素香
何斐%李煥麗%陳彊%郭素香
하비%리환려%진강%곽소향
普瑞巴林%神经病理性疼痛%背根神经节%p-JN K
普瑞巴林%神經病理性疼痛%揹根神經節%p-JN K
보서파림%신경병이성동통%배근신경절%p-JN K
Pregabalin%Neuropathic pain%Dorsal root ganglion%p-JNK
目的：观察不同剂量普瑞巴林对慢性坐骨神经压迫损伤所致神经病理性疼痛大鼠痛行为及背根神经节p-JN K表达的影响。方法雌性SD大鼠60只，随机分为5组，每组12只：假手术组（S组）、CCI组（C组）和不同剂量普瑞巴林 P1组、P2组、P3组。S组仅分离坐骨神经不结扎，C组和P1、P2、P3组建立CCI模型，P1、P2、P3组于术后第1天开始至取材时点分别胃内灌注普瑞巴林7 mg/kg、15 mg/kg、30 mg/kg ，S组和C组灌相同体积生理盐水。各组大鼠分别于术前1 d ，术后1、3、7、15天测定热痛阈PWL值，且在术后第3、7、15天测定热痛阈后每组随机取4只大鼠术侧背根神经节，免疫组化法测定背根神经节 p-JNK的表达。结果与术前1 d比较，C组、P1组术后1、3、7、15 d PWL值和p-JNK的表达有显著统计学差异（P＜0.05）。与C组比较，P1、P2、P3组PWL值和p-JNK在术后3、7、15 d的表达有显著性差异（P＜0.05）。与 P1组比较，P2、P3组术后1、3、7、15 d PWL值和p-JNK的表达差异有统计学意义（P＜0.05）。结论不同剂量普瑞巴林对CCI模型大鼠致神经病理性疼痛有镇痛作用且呈剂量依赖性，其机制可能与抑制p-JN K的活化有关。
目的：觀察不同劑量普瑞巴林對慢性坐骨神經壓迫損傷所緻神經病理性疼痛大鼠痛行為及揹根神經節p-JN K錶達的影響。方法雌性SD大鼠60隻，隨機分為5組，每組12隻：假手術組（S組）、CCI組（C組）和不同劑量普瑞巴林 P1組、P2組、P3組。S組僅分離坐骨神經不結扎，C組和P1、P2、P3組建立CCI模型，P1、P2、P3組于術後第1天開始至取材時點分彆胃內灌註普瑞巴林7 mg/kg、15 mg/kg、30 mg/kg ，S組和C組灌相同體積生理鹽水。各組大鼠分彆于術前1 d ，術後1、3、7、15天測定熱痛閾PWL值，且在術後第3、7、15天測定熱痛閾後每組隨機取4隻大鼠術側揹根神經節，免疫組化法測定揹根神經節 p-JNK的錶達。結果與術前1 d比較，C組、P1組術後1、3、7、15 d PWL值和p-JNK的錶達有顯著統計學差異（P＜0.05）。與C組比較，P1、P2、P3組PWL值和p-JNK在術後3、7、15 d的錶達有顯著性差異（P＜0.05）。與 P1組比較，P2、P3組術後1、3、7、15 d PWL值和p-JNK的錶達差異有統計學意義（P＜0.05）。結論不同劑量普瑞巴林對CCI模型大鼠緻神經病理性疼痛有鎮痛作用且呈劑量依賴性，其機製可能與抑製p-JN K的活化有關。
목적：관찰불동제량보서파림대만성좌골신경압박손상소치신경병이성동통대서통행위급배근신경절p-JN K표체적영향。방법자성SD대서60지，수궤분위5조，매조12지：가수술조（S조）、CCI조（C조）화불동제량보서파림 P1조、P2조、P3조。S조부분리좌골신경불결찰，C조화P1、P2、P3조건립CCI모형，P1、P2、P3조우술후제1천개시지취재시점분별위내관주보서파림7 mg/kg、15 mg/kg、30 mg/kg ，S조화C조관상동체적생리염수。각조대서분별우술전1 d ，술후1、3、7、15천측정열통역PWL치，차재술후제3、7、15천측정열통역후매조수궤취4지대서술측배근신경절，면역조화법측정배근신경절 p-JNK적표체。결과여술전1 d비교，C조、P1조술후1、3、7、15 d PWL치화p-JNK적표체유현저통계학차이（P＜0.05）。여C조비교，P1、P2、P3조PWL치화p-JNK재술후3、7、15 d적표체유현저성차이（P＜0.05）。여 P1조비교，P2、P3조술후1、3、7、15 d PWL치화p-JNK적표체차이유통계학의의（P＜0.05）。결론불동제량보서파림대CCI모형대서치신경병이성동통유진통작용차정제량의뢰성，기궤제가능여억제p-JN K적활화유관。
Objective To observe the effects of pregabalin on the pain behavior of neuropathic pain caused by chronic sciatic nerve injury and the p-JNK expression in dorsal root ganglia in rats. Methods 60 female SD rats were randomly divided into 5 groups ,namely sham-operation group (group S) ,pregabalin group (group P1 ,group P2 ,group P3) and chronic constrictive injury group (CCI group).In group S ,the right sciatic nerve was exposed but not ligated.Rats in pregabalin group and CCI group were received the chronic constrictive injury operation. Rats in group P1 ,group P2 and group P3 were performed the gas-tric perfusion with 7mg/kg ,15mg/kg and 30mg/kg of pregabalin ,respectively.Rats in group S and CCI group were received with the same volume of physiological saline. Thermal pain thresholds of rats in each group were measured by paw withdrawal latencies at 1d before operation and 3 ,7 and 15 d after operation. After thermal pain threshold detected at postoperative 3 ,7 and 15 d ,four rats in each group were randomly selected to perform CCI ,and then the p-JNK expression of DRG was detected with immunohistochemi-cal technique.Results The PWL values and the p-JNK expression in group P1 and group C at postop-erative 1 ,3 ,7 and 15 d were lower and higher than these preoperative 1 day ,respectively. The PWL values and the p-JNK ex-pression in group P1 ,P2 and P3 at postoperative 3 ,7 and 15 d were higher and lower than these in group C ,respectively. The PWL values and the p-JNK expression in group P2 and P3 at postoperative 1 ,3 ,7 and 15 d were higher and lower than these in group P1 ,respectively.Conclusion Pregabalin has the abirritation and dose-dependent characteristic effects on rats with neuropathic pain caused by CCI ,which maybe associated with inhibition of p-JNK activation.
