CAJ | 학술논문

目的：测定类风湿关节炎(RA)患者血清中IL-22的水平并分析其临床意义。方法采用ELISA法检测70例初诊的RA患者(RA组)和18例健康体检者(健康对照组)血清中IL-22的水平，其中RA组患者进一步分为RA活动组和RA非活动组，有骨侵蚀组和无骨侵蚀组，类风湿因子(RF)阳性组和RF阴性组，抗环瓜氨酸肽(抗CCP)抗体阳性组和抗CCP抗体阴性组。分析IL-22在不同组别的表达及其与RA患者临床及炎性指标的相关性。结果①RA组患者血清IL-22水平[(80.30±37.73)pg/mL]较健康对照组[(29.29±7.38)pg/mL]明显升高，差异有高度统计学意义(P<0.01)；其中RA活动组[(86.58±41.21)pg/mL]与RA非活动组[(63.24±17.98)pg/mL]及健康对照组[(29.29±7.38)pg/mL]比较，差异均有统计学意义(P<0.05)；RA组患者有骨侵蚀组[(94.71±42.20)pg/mL]与RA无骨侵蚀组[(56.03±18.16)pg/mL]比较，差异有统计学意义(P<0.05)；②IL-22在RF阳性组、抗CCP抗体阳性组[(84.65±38.47)、(100.3±43.68)pg/mL]明显高于RF阴性组及抗CCP抗体阴性组[(59.48±39.01)、(64.12±18.32)pg/mL]，差异均有统计学意义(P<0.05)；③RA组患者血清IL-22中水平与DAS28、RF呈正相关性(r =0.2883，P=0.0382；r=0.3670，P=0.0074)。结论 RA患者血清IL-22水平明显升高，可能参与了RA的发病机制，可作为RA病情活动及骨侵蚀的预测指标。
목적：측정류풍습관절염(RA)환자혈청중IL-22적수평병분석기림상의의。방법채용ELISA법검측70례초진적RA환자(RA조)화18례건강체검자(건강대조조)혈청중IL-22적수평，기중RA조환자진일보분위RA활동조화RA비활동조，유골침식조화무골침식조，류풍습인자(RF)양성조화RF음성조，항배과안산태(항CCP)항체양성조화항CCP항체음성조。분석IL-22재불동조별적표체급기여RA환자림상급염성지표적상관성。결과①RA조환자혈청IL-22수평[(80.30±37.73)pg/mL]교건강대조조[(29.29±7.38)pg/mL]명현승고，차이유고도통계학의의(P<0.01)；기중RA활동조[(86.58±41.21)pg/mL]여RA비활동조[(63.24±17.98)pg/mL]급건강대조조[(29.29±7.38)pg/mL]비교，차이균유통계학의의(P<0.05)；RA조환자유골침식조[(94.71±42.20)pg/mL]여RA무골침식조[(56.03±18.16)pg/mL]비교，차이유통계학의의(P<0.05)；②IL-22재RF양성조、항CCP항체양성조[(84.65±38.47)、(100.3±43.68)pg/mL]명현고우RF음성조급항CCP항체음성조[(59.48±39.01)、(64.12±18.32)pg/mL]，차이균유통계학의의(P<0.05)；③RA조환자혈청IL-22중수평여DAS28、RF정정상관성(r =0.2883，P=0.0382；r=0.3670，P=0.0074)。결론 RA환자혈청IL-22수평명현승고，가능삼여료RA적발병궤제，가작위RA병정활동급골침식적예측지표。
Objective To test the serum level of IL-22 in patients with rheumatoid arthritis(RA) and analyze its clinical significance. Methods Concentrations of IL-22 in the serum of 70 newly diagnosed RA patients (RA group) and 18 healthy controls (healthy control group) were measured by enzyme-linked immunosorbent assay. Patients of RA group were further divided into active RA group and non-active RA group, bone erosions group and without bone erosions group, rheumatoid factor (RF) positivity group and RF negative group, anti-cyclic citrullinated peptide antibodies (anti-CCP) positive group and anti-CCP negative group. The expression of IL-22 in different groups and the correlation be-tween IL-22 and the clinical inflammatory indexes of RA patients were analyzed. Results ①Serum IL-22 content in RA group [(80.30±37.73) pg/mL] were significantly higher than that of healthy control group [(29.29±7.38) pg/mL], the difference was statistically significant (P< 0.01). There was a significant difference between active RA group [(86.58±41.21) pg/mL], non-active RA group [(80.30±37.73) pg/mL] and healthy control group [(29.29±7.38) pg/mL], the differ-ences were statistically significant (P< 0.05). There was a significant difference between bone erosions group [(94.71±42.20) pg/mL] and without bone erosions group [(56.03±18.16) pg/mL] (P<0.05);②IL-22 in the RF positive group and anti-CCP antibody positive group [(84.65±38.47), (100.3±43.68) pg/mL] were higher than those of RF negative groups and anti-CCP antibody negative group [(59.48±39.01), (64.12±18.32) pg/mL], the differences were statistically significant (P<0.05);③the levels of serum IL-22 in RA patients were positive related with disease activity index DAS28 and RF (r=0.2883, P=0.0382;r=0.3670, P=0.0074). Conclusion The significantly elevated IL-22 in the serum of patients with es-tablished RA indicates that IL-22 may be involved in the pathogenesis of RA, and can be used as a predictor for the disease activity and bone erosion of RA.