CAJ | 학술논문

目的：探讨成功建立大鼠局灶性脑缺血模型的有效方法及影响因素。方法将大鼠分为250~300 g和350~400 g两个体重组。为观察禁食对造模的影响，250~300 g体重组又分为对照组(n=10)和禁食组(n=10)；为观察栓线直径对造模的影响，350~400 g体重组又分为细栓线组(n=10)和粗栓线组(n=10)。栓线经颈总动脉、颈内动脉进入大脑中动脉的起始部位阻断大脑中动脉的血流。术后通过神经功能缺损评分和氯化三苯基四氮唑(TTC)染色对该模型进行评价。结果在250~300 g体重组，造模成功率高，禁食组死亡率(11.1%)明显低于对照组(62.5%)(P<0.05)；在350~400 g体重组，死亡率低，粗栓线组造模成功率(80%)明显高于细栓线组(20%)(P<0.05)。结论术前禁食可降低动物死亡率，根据大鼠体重选择相应直径的栓线可提高造模的成功率。
목적：탐토성공건립대서국조성뇌결혈모형적유효방법급영향인소。방법장대서분위250~300 g화350~400 g량개체중조。위관찰금식대조모적영향，250~300 g체중조우분위대조조(n=10)화금식조(n=10)；위관찰전선직경대조모적영향，350~400 g체중조우분위세전선조(n=10)화조전선조(n=10)。전선경경총동맥、경내동맥진입대뇌중동맥적기시부위조단대뇌중동맥적혈류。술후통과신경공능결손평분화록화삼분기사담서(TTC)염색대해모형진행평개。결과재250~300 g체중조，조모성공솔고，금식조사망솔(11.1%)명현저우대조조(62.5%)(P<0.05)；재350~400 g체중조，사망솔저，조전선조조모성공솔(80%)명현고우세전선조(20%)(P<0.05)。결론술전금식가강저동물사망솔，근거대서체중선택상응직경적전선가제고조모적성공솔。
Objective To investigate the method and influence factors of establishing focal ischemia model of rat suc-cessfully. Methods Rats were divided into two groups, 250-300 g group and 350-400 g group. In order to observe the influence of fasting on the model, 250-300 g group was subdivided into two groups, control group (n=10) and fasting group (n=10). In order to observe the influence of the bolt line diameter on the model, 350-400 g group was sub-divid-ed into two groups, thin bolt line group (n=10) and bold bolt line group (n=10). The bolt line entered from a cut of the common carotid artery and moved in the intracranial internal carotid artery and at last reached to the starting positions of middle cerebral artery to block blood flow. The nerve function defect score and TTC staining were used to evaluate the model. Results In the 250-300 g group, the success rate was high and mortality of the fasting group (11.1%) was lower than the control group (62.5%) (P<0.05). In the 350-400 g group, mortality was low and the success rate of the bold bolt line group (80%) was higher than the thin bolt line group (20%) (P< 0.05). Conclusion Fasting can reduce animal mortality. Selecting the bolt line according to the weight of rats can increase success rate.