CAJ | 학술논문

目的：调查烧伤患者耐甲氧西林金黄色葡萄球菌(MRSA)的耐药性及分子流行病学情况,为控制感染提供科学依据。方法烧伤患者分离的金黄色葡萄球菌用PCR法扩增其mecA基因确定MRSA菌株,K-B纸片法检测MRSA的耐药性,随机引物扩增多态性DNA(RAPD)进行同源性分析。结果 MRSA的分离率为41.1%,对多种抗生素均耐药,没有发现耐万古霉素和替考拉宁的菌株。23株MRSA经RAPD分型,均获得稳定的条带,可分为四型,以Ⅱ型为主,共检出13株。结论烧伤患者分离MRSA菌株具有多重耐药性,应根据药敏试验结果合理选用抗生素进行治疗；采用RAPD对MRSA的同源性进行研究,为控制感染提供分子流行病学依据。
목적：조사소상환자내갑양서림금황색포도구균(MRSA)적내약성급분자류행병학정황,위공제감염제공과학의거。방법소상환자분리적금황색포도구균용PCR법확증기mecA기인학정MRSA균주,K-B지편법검측MRSA적내약성,수궤인물확증다태성DNA(RAPD)진행동원성분석。결과 MRSA적분리솔위41.1%,대다충항생소균내약,몰유발현내만고매소화체고랍저적균주。23주MRSA경RAPD분형,균획득은정적조대,가분위사형,이Ⅱ형위주,공검출13주。결론소상환자분리MRSA균주구유다중내약성,응근거약민시험결과합리선용항생소진행치료；채용RAPD대MRSA적동원성진행연구,위공제감염제공분자류행병학의거。
Objective To investigate the status of antibiotics resistance and the genetic homology of methicil in-resistant staphylococcus aureus isolated from burn wounds and provide scientific evidence for control ing MRSA infection. Methods MRSA was identified by mecA PCR. Drug resistance was perfomed using K-B method. The RAPD technique was used to identify the genotypes. Results The separation rate of MRSA was 41.1%. MRSA was resistant to much drugs .But al strains were susceptible to vancomycin and teicoplanin. All 23 stains of MRSA produced fingerprints by RAPD, and was classified into four genotypes.13 strains wereIItype which was the dominant type. Conclusion The MRSA isolated from burn wounds has high and multi drug resistance, we should resanobaly choose drugs according to the susceptibility test. RAPD results reveal the special epidemic genotypes of MRSA and this study can provide molecular epidemiological evidence for control of MRSA infection.