CAJ | 학술논문

背景：脂肪间充质干细胞是否是治疗因毛细胞退化、缺失所造成的感音神经性聋的福音呢？目的：探讨豚鼠脂肪间充质干细胞经耳蜗鼓阶途径植入感音神经性耳聋动物模型后对听力的修复作用。方法：庆大霉素腹腔注射建立豚鼠感音神经性耳聋动物模型，耳蜗鼓阶途径植入豚鼠脂肪间充质干细胞，分别于植入后1，3周检测听性脑干反应，观察植入脂肪间充质干细胞后耳聋动物听力的变化；并追踪EDU标记的豚鼠脂肪间充质干细胞在耳蜗内的迁移及分布情况。结果与结论：在植入后1周及3周进行听性脑干反应检测，听力较移植前逐渐好转。植入细胞后1周，细胞大多分布在外淋巴液中，部分迁移至耳蜗柯蒂器贴附于基底膜上，植入细胞后3周，细胞不仅迁移并贴附在Corti器基底膜发挥作用，而且部分迁移到蜗神经，植入时间越长，存活细胞越少。结果表明豚鼠脂肪间充质干细胞通过耳蜗鼓阶途径微孔植入，可以定向迁移并存活最终达到提高听力的目的。
배경：지방간충질간세포시부시치료인모세포퇴화、결실소조성적감음신경성롱적복음니？목적：탐토돈서지방간충질간세포경이와고계도경식입감음신경성이롱동물모형후대은력적수복작용。방법：경대매소복강주사건립돈서감음신경성이롱동물모형，이와고계도경식입돈서지방간충질간세포，분별우식입후1，3주검측은성뇌간반응，관찰식입지방간충질간세포후이롱동물은력적변화；병추종EDU표기적돈서지방간충질간세포재이와내적천이급분포정황。결과여결론：재식입후1주급3주진행은성뇌간반응검측，은력교이식전축점호전。식입세포후1주，세포대다분포재외림파액중，부분천이지이와가체기첩부우기저막상，식입세포후3주，세포불부천이병첩부재Corti기기저막발휘작용，이차부분천이도와신경，식입시간월장，존활세포월소。결과표명돈서지방간충질간세포통과이와고계도경미공식입，가이정향천이병존활최종체도제고은력적목적。
BACKGROUND:Can adipose-derived mesenchymal stem cells be used to treat sensorineural deafness caused by hair celldegeneration and loss? OBJECTIVE:To investigate the effect of adipose-derived mesenchymal stem cells from guinea pigs transplanted via the scala tympani on the recovery from sensorineural hearing in an animal model. METHODS:Intraperitoneal injection of gentamicin was performed to prepare sensorineural deafness models in guinea pigs. Then, adipose-derived mesenchymal stem cells from guinea pig were transplanted via the scala tympani. After 1 and 3 weeks, auditory brainstem responses were detected to observe the hearing variation after adipose-derived mesenchymal stem celltransplantation. Meanwhile, the migration and distribution of EDU-labeled adipose-derived mesenchymal stem cells in the cochlea were traced. RESULTS AND CONCLUSION:After 1 and 3 weeks of implantation, the results from auditory brainstem response showed that the hearing was gradual y improved. At 1 week of implantation, most of the cells were distributed in the perilymph and some cells migrated to the Corti organ and attached to the basement membrane. At 3 weeks of implantation, cells migrated and attached to the basement membrane of Corti organ, furthermore, some cells migrated to the cochlear nerve. The longer the time of implantation, the less cellsurvived. The results indicate that adipose-derived mesenchymal stem cells from guinea pig that are transplanted via the scala tympani can directly migrate and survive ultimately to improve the hearing.