中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
32期
5166-5172
,共7页
干细胞%脐带脐血干细胞%肝干细胞%脐带间充质干细胞%生长曲线%倍增时间
榦細胞%臍帶臍血榦細胞%肝榦細胞%臍帶間充質榦細胞%生長麯線%倍增時間
간세포%제대제혈간세포%간간세포%제대간충질간세포%생장곡선%배증시간
umbilical cord%liver%mesenchymal stem cells%growth charts
背景：不同来源的干细胞具有不同的分子特征及生长特性，对疾病治疗的机制及效果可能会有所差异。目的：比较两种来源间充质干细胞的生物学特性。方法：分离培养人脐带源、胚胎肝来源的间充质干细胞，传至第5代时分别进行细胞形态观察，细胞群体倍增时间计算，细胞表面标志鉴定，诱导分化能力检测。另外将脐带源间充质干细胞继续培养至第10，15代，绘制生长曲线并计算细胞群体倍增时间。结果与结论：两种来源的间充质干细胞在对数生长期均为梭形，旋涡状生长，均具有成骨、成脂、成软骨分化的能力。两种间充质干细胞的生长曲线均为S形。第5代胎肝来源间充质干细胞的倍增时间为(34.37±0.31) h，脐带源间充质干细胞的倍增时间为(35.63±0.38) h，差异无显著性意义(P>0.05)。第10代和第15代脐带源间充质干细胞的倍增时间分别为(52.6±0.53) h和(53.27±0.92) h，与第5代比较差异有显著性意义(P<0.05)。结果可见两种来源间充质干细胞生物学特性基本相同，肉眼观察胎肝来源间充质干细胞比脐带源间充质干细胞形态稍小，增殖较快，但统计学上差异无显著性意义。
揹景：不同來源的榦細胞具有不同的分子特徵及生長特性，對疾病治療的機製及效果可能會有所差異。目的：比較兩種來源間充質榦細胞的生物學特性。方法：分離培養人臍帶源、胚胎肝來源的間充質榦細胞，傳至第5代時分彆進行細胞形態觀察，細胞群體倍增時間計算，細胞錶麵標誌鑒定，誘導分化能力檢測。另外將臍帶源間充質榦細胞繼續培養至第10，15代，繪製生長麯線併計算細胞群體倍增時間。結果與結論：兩種來源的間充質榦細胞在對數生長期均為梭形，鏇渦狀生長，均具有成骨、成脂、成軟骨分化的能力。兩種間充質榦細胞的生長麯線均為S形。第5代胎肝來源間充質榦細胞的倍增時間為(34.37±0.31) h，臍帶源間充質榦細胞的倍增時間為(35.63±0.38) h，差異無顯著性意義(P>0.05)。第10代和第15代臍帶源間充質榦細胞的倍增時間分彆為(52.6±0.53) h和(53.27±0.92) h，與第5代比較差異有顯著性意義(P<0.05)。結果可見兩種來源間充質榦細胞生物學特性基本相同，肉眼觀察胎肝來源間充質榦細胞比臍帶源間充質榦細胞形態稍小，增殖較快，但統計學上差異無顯著性意義。
배경：불동래원적간세포구유불동적분자특정급생장특성，대질병치료적궤제급효과가능회유소차이。목적：비교량충래원간충질간세포적생물학특성。방법：분리배양인제대원、배태간래원적간충질간세포，전지제5대시분별진행세포형태관찰，세포군체배증시간계산，세포표면표지감정，유도분화능력검측。령외장제대원간충질간세포계속배양지제10，15대，회제생장곡선병계산세포군체배증시간。결과여결론：량충래원적간충질간세포재대수생장기균위사형，선와상생장，균구유성골、성지、성연골분화적능력。량충간충질간세포적생장곡선균위S형。제5대태간래원간충질간세포적배증시간위(34.37±0.31) h，제대원간충질간세포적배증시간위(35.63±0.38) h，차이무현저성의의(P>0.05)。제10대화제15대제대원간충질간세포적배증시간분별위(52.6±0.53) h화(53.27±0.92) h，여제5대비교차이유현저성의의(P<0.05)。결과가견량충래원간충질간세포생물학특성기본상동，육안관찰태간래원간충질간세포비제대원간충질간세포형태초소，증식교쾌，단통계학상차이무현저성의의。
BACKGROUND:Different sources of stem cells have different molecular characteristics and growth characteristics;therefore, there are some differences in therapeutic mechanisms and effects. OBJECTIVE:To compare mesenchymal stem cells growth characteristics form two sources. METHODS:Mesenchymal stem cells from the umbilical cord and the embryonic liver were isolated and cultured. Passage 5 cells were used to observe the cellmorphology, calculate the doubling time of cellpopulation-doubling time, identify surface markers and determine the differentiation capacity. Mesenchymal stem cells from the umbilical cord were subcultured to passages 10 and 15, and cellcurves were drawn and population doubling time was calculated. RESULTS AND CONCLUSION:Mesenchymal stem cells from these two sources in logarithmic phase were fusiform and grew spiral y with osteogenic, adipogenic, and chondrogenic capacities. The growth curves of cells were both S-shaped. At passage 5, the doubling time was (34.37±0.31) hours for embryonic liver-derived mesenchymal stem cells and (35.63±0.38) hours for umbilical cord-derived mesenchymal stem cells, and there was no significant difference (P>0.05). However, the population doubling time of passages 10 and 15 umbilical cord-derived mesenchymal stem cells was (52.6±0.53) and (53.27±0.92) hours, respectively, which was significantly difference from that of passage 5 cells (P<0.05). The cellmorphology and growth curve from two sources are basical y the same. Embryonic liver-derived stem cells are smal er and proliferate faster than umbilical cord-derived mesenchymal stem cells, but no statistical difference is found between the two types.
