CAJ | 학술논문

背景：既往依达拉奉作为抗氧化剂对神经细胞氧化损伤的保护作用已得到证实，但其对骨髓基质干细胞氧化损伤的保护作用未见明确报道及深入研究。目的：观察依达拉奉对骨髓基质干细胞在氧化损伤中的调节作用。方法：通过冲洗髓腔的方法提取新西兰大耳白兔的长骨骨髓，然后应用密度梯度离心联合贴壁筛选的方法体外培养获得骨髓基质干细胞。实验将第3代骨髓基质干细胞分为5组：空白组仅加入体积分数为10%胎牛血清、1%的双抗的低糖DMEM培养液；地塞米松组加入含有1×10-7 mol/L地塞米松的细胞培养液，不含有依达拉奉；50，100，300 mg/L依达拉奉组分别加入1×10-7 mol/L的地塞米松和质量浓度为50，100，300 mg/L的依达拉奉，培养后分别用四甲基偶氮唑蓝法、流式细胞仪法检测细胞增殖水平及细胞周期。结果与结论：依达拉奉组较空白组及地塞米松组细胞增殖水平明显增强，依达拉奉对骨髓基质干细胞起到了保护作用，当依达拉奉质量浓度为50 mg/L时即发挥作用(P<0.05)，并且与依达拉奉的质量浓度有一定的量效关系，当依达拉奉质量浓度为100 mg/L时，其保护作用明显提高(P<0.01)，但随着质量浓度的增加，这种保护作用没有进一步增加，反而稍有下降。结果表明：高浓度地塞米松可以使骨髓基质干细胞受到氧化损伤，依达拉奉可以通过抗氧化作用保护骨髓基质干细胞免受氧化损伤。
배경：기왕의체랍봉작위항양화제대신경세포양화손상적보호작용이득도증실，단기대골수기질간세포양화손상적보호작용미견명학보도급심입연구。목적：관찰의체랍봉대골수기질간세포재양화손상중적조절작용。방법：통과충세수강적방법제취신서란대이백토적장골골수，연후응용밀도제도리심연합첩벽사선적방법체외배양획득골수기질간세포。실험장제3대골수기질간세포분위5조：공백조부가입체적분수위10%태우혈청、1%적쌍항적저당DMEM배양액；지새미송조가입함유1×10-7 mol/L지새미송적세포배양액，불함유의체랍봉；50，100，300 mg/L의체랍봉조분별가입1×10-7 mol/L적지새미송화질량농도위50，100，300 mg/L적의체랍봉，배양후분별용사갑기우담서람법、류식세포의법검측세포증식수평급세포주기。결과여결론：의체랍봉조교공백조급지새미송조세포증식수평명현증강，의체랍봉대골수기질간세포기도료보호작용，당의체랍봉질량농도위50 mg/L시즉발휘작용(P<0.05)，병차여의체랍봉적질량농도유일정적량효관계，당의체랍봉질량농도위100 mg/L시，기보호작용명현제고(P<0.01)，단수착질량농도적증가，저충보호작용몰유진일보증가，반이초유하강。결과표명：고농도지새미송가이사골수기질간세포수도양화손상，의체랍봉가이통과항양화작용보호골수기질간세포면수양화손상。
BACKGROUND:Edaravone as an antioxidant protective effect on nerve cells injured by hydrogen peroxide has been confirmed, but its protective effect on oxidative damage to bone marrow stromal cells has not been reported in-depth. OBJECTIVE:To investigate the regulatory effects of edaravone on oxidative injury to bone marrow stromal cells. METHODS:Bone marrow samples were extracted from the long bone of New Zealand rabbits by the method of washing the pulp cavity, then subjected to the density gradient centrifugation and adherent screening to obtain bone marrow stromal stem cells in vitro. The bone marrow stromal cells at 3 passage were divided into five groups:blank group, treated with low-glucose Dulbecco’s modified Eagle’s medium containing 10%fetal bovine serum and 1%double antibody;dexamethasone group, treated with cellculture medium containing 1×10-7 mol/L dexamethasone;50, 100, 300 mg/L edaravone groups, cultured in cellculture medium containing 1×10-7 mol/L dexamethasone and 50, 100, 300 mg/L edaravone, respectively. After culture, MTT method and flow cytometry were used to detect the proliferative level and cellcycle of cells. RESULTS AND CONCLUSION:Compared with the control and dexamethasone groups, edaravone significantly enhanced the cellproliferation. Edaravone played a protective role in bone marrow stromal cells. When the concentration was 50 mg/L, edaravone began to play a regulatory role (P<0.05), and this effect was certainly associated with the concentration of edaravone. When the concentration was up to 100 mg/L, edaravone showed a better protective role (P<0.01). However, with increasing concentration, this protective effect was not further increased, but decreased slightly. Results indicated that high-concentration dexamethasone can induce oxidative injury to bone marrow stromal cells, and edaravone can protect the cells against this oxidative damage by antioxidant role.