CAJ | 학술논문

目的：研究番石榴叶总黄酮抑制泡沫细胞形成及 LOX-1表达的作用。方法建立 T HP-1单核-巨噬细胞源性泡沫细胞模型，根据CCK-8法测定的番石榴叶总黄酮对该细胞的安全浓度范围，将配制的番石榴总黄铜溶液在其安全范围内选取低、中、高三个剂量组，以无血清培养液的空白对照组及ox-LDL 模型组为对照。采用油红O染色观察细胞内脂质堆积情况，酶偶联比色法检测细胞内总胆固醇含量，Western blot法检测 LOX-1的蛋白表达水平。结果三个剂量组细胞内总胆固醇含量均低于模型组（P＜0.05），并随番石榴叶总黄酮浓度升高而下降；三个剂量组的 LOX-1蛋白表达均低于模型组（P＜0.05），抑制作用随剂量升高而增强。结论番石榴叶总黄酮通过抑制 LOX-1的表达，减少巨噬细胞摄取ox-LDL ，抑制泡沫细胞形成，从而起到抗动脉粥样硬化的作用。
목적：연구번석류협총황동억제포말세포형성급 LOX-1표체적작용。방법건립 T HP-1단핵-거서세포원성포말세포모형，근거CCK-8법측정적번석류협총황동대해세포적안전농도범위，장배제적번석류총황동용액재기안전범위내선취저、중、고삼개제량조，이무혈청배양액적공백대조조급ox-LDL 모형조위대조。채용유홍O염색관찰세포내지질퇴적정황，매우련비색법검측세포내총담고순함량，Western blot법검측 LOX-1적단백표체수평。결과삼개제량조세포내총담고순함량균저우모형조（P＜0.05），병수번석류협총황동농도승고이하강；삼개제량조적 LOX-1단백표체균저우모형조（P＜0.05），억제작용수제량승고이증강。결론번석류협총황동통과억제 LOX-1적표체，감소거서세포섭취ox-LDL ，억제포말세포형성，종이기도항동맥죽양경화적작용。
Objective To investigate the effect of total flavones from Psidium guajava leaves on the formation of macrophage foam cell and the expression of LOX-1 protein.Methods THP-1 was incubated with ox-LDL to es-tablish the model of macrophage foam cell formation.The proper concentrations of total flavones were determined by CCK-8.The intracellular lipid deposition was examined by oil red staining.The intracellular total cholesterol (TC)was measured by the enzymatic method.The expression of LOX-1 protein was examined by Western blot.Results Compared with model group ,the intracellular TC content of all the total flavones groups were lower than that of ox-LDL group(P<0.05).Furthermore ,TC content was decreased with the increase of total flavones con-centration.Meanwhile ,the expression of LOX-1 protein in three flavones groups was significantly reduced (P<0.05)in a dose-dependent manner similar to that of TC.Conclusion Total flavones from Psidium guajava may re-duce ox-LDL uptake of macrophages w hich can inhibit foam cells formation and LOX-1 protein expression.