CAJ | 학술논문

【目的】研究丙酮/（NH4）2 SO4双水相萃取体系对分离槐米中芦丁的影响因素。【方法】利用正交设计法进行优化，并把28．57％的硫酸铵溶液与等体积的70％的丙酮混合溶液，调至 pH＝9形成的双水相，两相的体积比（V上/V下）是1．62。【结果】萃取体系的上相中芦丁含量达到95．92％。【结论】丙酮/（NH4）2SO4双水相萃取体系分离芦丁具有良好的选择性。
【목적】연구병동/（NH4）2 SO4쌍수상췌취체계대분리괴미중호정적영향인소。【방법】이용정교설계법진행우화，병파28．57％적류산안용액여등체적적70％적병동혼합용액，조지 pH＝9형성적쌍수상，량상적체적비（V상/V하）시1．62。【결과】췌취체계적상상중호정함량체도95．92％。【결론】병동/（NH4）2SO4쌍수상췌취체계분리호정구유량호적선택성。
Objective]Study on the influence factors of Rutin Extraction from Flos Sophorae Immaturus by acetone?ammonium sulfate aqueous two?phase system.[Methods]The tech-nique conditions was optimized by orthogond test.The aqueous two-phase system (pH 9.0) consisted of aqueous (NH4)2SO4solution (28.57%,W%)and acetone (70%,W%)in equal volume,and ratio of volume of two phases (Vl/Vh ,Vl is light phase,Vh is heavy phase)is 1.62.[Results]By this extract system,the content of rutin in light phase is 95.92%.[Conclu-sion]This result revealed that the aqueous liquid two?phase system was effective for separa-ting rutin.