CAJ | 학술논문

目的：研究氯化镧短期重复染毒对ICR小鼠产生的遗传毒性和氧化损伤；方法：ICR小鼠随机分为对照组、低剂量组和高剂量组，分别给予0 mg/kg、20 mg/kg、200 mg/kg氯化镧，连续灌胃7 d，测定外周血淋巴细胞微核，取脏器分别测定丙二醛（ malondiadehyde，MDA）、谷胱甘肽（ glutathione，GSH）、超氧化物歧化酶（ SuperoXide dismutase，SOD）的含量。结果：高剂量组微核率显著高于对照组（ P <0.05）；镧元素在心脏、肝脏、脾脏和肾脏的蓄积量，高剂量组与低剂量组和对照组相比差异均有统计学意义（ P <0.05）；高剂量组小鼠脾脏、肾脏组织中MDA含量高于对照组（ P <0.05）；高剂量组小鼠脾脏、肾脏中 GSH、T -SOD 含量低于低剂量组和对照组（ P <0.05）。结论：氯化镧经口染毒剂量在200 mg/kg时可在各脏器中蓄积，且可引起小鼠脏器发生氧化损伤和骨髓微核率的增加，提示200 mg/kg氯化镧对小鼠有一定的遗传毒性，氧化损伤可能是其引起遗传毒性的机制之一。
목적：연구록화란단기중복염독대ICR소서산생적유전독성화양화손상；방법：ICR소서수궤분위대조조、저제량조화고제량조，분별급여0 mg/kg、20 mg/kg、200 mg/kg록화란，련속관위7 d，측정외주혈림파세포미핵，취장기분별측정병이철（ malondiadehyde，MDA）、곡광감태（ glutathione，GSH）、초양화물기화매（ SuperoXide dismutase，SOD）적함량。결과：고제량조미핵솔현저고우대조조（ P <0.05）；란원소재심장、간장、비장화신장적축적량，고제량조여저제량조화대조조상비차이균유통계학의의（ P <0.05）；고제량조소서비장、신장조직중MDA함량고우대조조（ P <0.05）；고제량조소서비장、신장중 GSH、T -SOD 함량저우저제량조화대조조（ P <0.05）。결론：록화란경구염독제량재200 mg/kg시가재각장기중축적，차가인기소서장기발생양화손상화골수미핵솔적증가，제시200 mg/kg록화란대소서유일정적유전독성，양화손상가능시기인기유전독성적궤제지일。
ObjectiVe:To inVestigate the genetic toXicity and oXidatiVe damage caused by short-term-repeated eXposure to lanthanum chloride in ICR mice. Methods:ICR mice were randomly diVided into the control group,the low-dose lanthanum chloride group and the high dose- lanthanum chloride group. The mice in the three groups were then giVen by gaVage 0 mg/kg, 20 mg/kg,and 200 mg/kg of lanthanum chloride,respectiVely,for 7 days consecutiVely. Micronucleus frequency in the periph-eral lymphocytes,as well as MDA,GSH and SOD in the tissues was determined. ResuIts:Micronucleus frequency in the high-dose group was significantly higher than that in the control group( P <0. 05). There was statistically significant difference in the stock Volume of lanthanum in the heart,liVer,spleen and kidney among high-dose,low-dose group and the control group (P < 0· 05). MDA content in the spleen and kidney of the mice in the high-dose group was also significantly higher than that in the control group( P <0. 05),while GSH and T-SOD contents in the spleen and kidney of the mice in the high- dose of lanthanum chloride were lower,compared with the low-dose group and the control group( P <0. 05). ConcIusion:Lanthanum chloride may deposit in eVery organ when it reaches the amount of 200 mg/kg per os,resulting in oXidatiVe damage to mice organs and increases in micronucleus rate. There is a hint that 200 mg / kg of lanthanum chloride could bring about genetic toXicity to certain degree on mice,and oXidatiVe damage may be one of the mechanisms causing genetic toXicity.