包头医学院学报
包頭醫學院學報
포두의학원학보
JOURNAL OF BAOTOU MEDICAL COLLEGE
2014年
4期
3-5
,共3页
枸杞叶%桑叶%水提取物%体外抑菌试验%最小抑菌浓度
枸杞葉%桑葉%水提取物%體外抑菌試驗%最小抑菌濃度
구기협%상협%수제취물%체외억균시험%최소억균농도
Wolfberry leaf%mulberry leaf%Aqueous eXtract%Bacteriostatic test in Vitro%Minimum bacteriostatic concentra-tion(MIC)
目的：研究枸杞叶、桑叶水提物对常见的病原微生物的体外抑菌作用。方法：将枸杞叶和桑叶提取物用试管二倍稀释法联合琼脂平板法，进行体外抑菌试验。结果：枸杞叶水提物对金黄色葡萄球菌最小抑菌浓度（ MIC ）为10 mg/mL，对表皮葡萄球菌MIC为20 mg/mL，对大肠杆菌、铜绿假单胞菌、白假丝酵母菌MIC均>20 mg/mL；桑叶水提取物对金黄色葡萄球菌的MIC为20 mg/mL；对表皮葡萄球菌、大肠杆菌、绿脓杆菌、白色念珠菌MIC均>20 mg/mL。结论：枸杞叶和桑叶水提物对金黄色葡萄球菌有抑菌作用，两者对革兰阴性菌、念珠菌抑菌作用不显著。
目的：研究枸杞葉、桑葉水提物對常見的病原微生物的體外抑菌作用。方法：將枸杞葉和桑葉提取物用試管二倍稀釋法聯閤瓊脂平闆法，進行體外抑菌試驗。結果：枸杞葉水提物對金黃色葡萄毬菌最小抑菌濃度（ MIC ）為10 mg/mL，對錶皮葡萄毬菌MIC為20 mg/mL，對大腸桿菌、銅綠假單胞菌、白假絲酵母菌MIC均>20 mg/mL；桑葉水提取物對金黃色葡萄毬菌的MIC為20 mg/mL；對錶皮葡萄毬菌、大腸桿菌、綠膿桿菌、白色唸珠菌MIC均>20 mg/mL。結論：枸杞葉和桑葉水提物對金黃色葡萄毬菌有抑菌作用，兩者對革蘭陰性菌、唸珠菌抑菌作用不顯著。
목적：연구구기협、상협수제물대상견적병원미생물적체외억균작용。방법：장구기협화상협제취물용시관이배희석법연합경지평판법，진행체외억균시험。결과：구기협수제물대금황색포도구균최소억균농도（ MIC ）위10 mg/mL，대표피포도구균MIC위20 mg/mL，대대장간균、동록가단포균、백가사효모균MIC균>20 mg/mL；상협수제취물대금황색포도구균적MIC위20 mg/mL；대표피포도구균、대장간균、록농간균、백색념주균MIC균>20 mg/mL。결론：구기협화상협수제물대금황색포도구균유억균작용，량자대혁란음성균、념주균억균작용불현저。
ObjectiVes:To identify the antibacterial actiVity of aqueous eXtract from wolfberry leaf and mulberry leaf against common pathogenic microorganisms in Vitro. Methods:The antibacterial actiVity of aqueous eXtract from wolfberry leaf and mul-berry leaf was identified by AGAR plate method and tube double dilution method. ResuIts:The minimum bacteriostatic concen-tration( MIC)of wolfberry leaf aqueous eXtract on Staphylococcus aureus and Epidermis staphylococcus was 10 mg/mL and 20 mg/mL respectiVely. The MIC of wolfberry leaf aqueous eXtract on Escherichia coli,Pseudomonas aeruginosa or White candi-da was greater than 20 mg/mL. Besides,the MIC of mulberry leaVes eXtract on Staphylococcus aureus was 20 mg/mL and The MIC on Epidermis staphylococcus,Escherichia coli,Pseudomonas aeruginosa or Candida albicans was greater than 20 mg/mL. ConcIusion:The minimum bacteriostatic concentration( MIC)of wolfberry leaf eXtract on Staphylococcus aureus was 10 mg/mL, The minimum bacteriostatic concentration( MIC)of mulberry leaf aqueous eXtract on Staphylococcus aureus was 20 mg/mL. The effect of both on gram-negatiVe bacteria,candida was not significant.
