CAJ | 학술논문

建立一种梯度洗脱反相高效液相色谱(H P LC )-二极管阵列检测器测定糕点中丙酸钙的方法。色谱柱为C18(4.6mm x150mm ,5μm )，流动相为1.5g/L的磷酸氢二铵酸性条件，在不同检测波长下进行检测，能够进行定性和定量的研究。结果表明，丙酸钙的出峰时间约为3.5m i n，样品检出限为0.03g/k g，线性回归方程相关系数r2=0.99990，方法回收率90%-101%。所建立的方法简单快速准确，实用性强。
건립일충제도세탈반상고효액상색보(H P LC )-이겁관진렬검측기측정고점중병산개적방법。색보주위C18(4.6mm x150mm ,5μm )，류동상위1.5g/L적린산경이안산성조건，재불동검측파장하진행검측，능구진행정성화정량적연구。결과표명，병산개적출봉시간약위3.5m i n，양품검출한위0.03g/k g，선성회귀방정상관계수r2=0.99990，방법회수솔90%-101%。소건립적방법간단쾌속준학，실용성강。
A method was established to determine calcium propionate in cake by a gradient RP highperformance liquid chromatography ( HPLC) with diode arraydetector. The compound was separated by a C18(4.6mm×150m m , 5μm)column with mobile phase of 1.5g/L acid ammonium phosphate, at different detection wavelength. Its qualitative and quantitative tests in this method have been done The experimental results show that calcium propionate was appeared in 3.5 min ,and the detection limit was 0.03g/kg, and the correlation coefifcient r2 of equation of linear regression was 0.99990,and recoveries were between 90%and 101%.The established method is rapid accurate with good practical application and simple pretreatment.