河南科技学院学报（自然科学版）
河南科技學院學報（自然科學版）
하남과기학원학보（자연과학판）
JOURNAL OF HENAN INSTITUTE OF SCIENCE AND TECHNOLOGY(NATURAL SCIENCES EDITION)
2014年
4期
16-20
,共5页
马俊洋%刘玲歌%吴星星%孙春然%郭聪敏%崔茂金%祝勇
馬俊洋%劉玲歌%吳星星%孫春然%郭聰敏%崔茂金%祝勇
마준양%류령가%오성성%손춘연%곽총민%최무금%축용
麦秆%纤维素酶%酶解%总还原糖%单因素试验%正交试验
麥稈%纖維素酶%酶解%總還原糖%單因素試驗%正交試驗
맥간%섬유소매%매해%총환원당%단인소시험%정교시험
wheat straw%cellulase%enzymolysis%total reducing sugars%single factor experiment%orthogonal experiments
以稀硫酸预处理的麦秆为底物,通过单因素试验和正交试验优化了底物的酶解条件.结果表明：酶解条件的主次顺序为底物质量浓度、酶质量浓度、酶解时间、酶解温度和pH；最佳酶解条件为底物质量浓度0.25 g/L、酶质量浓度2 g/L、酶解时间36 h、酶解温度45℃和pH为5.0,此时总还原糖含量最大为707.07 mg/g,糖含量显著增加.酶解对于提高麦秆的糖化率是非常有必要的.
以稀硫痠預處理的麥稈為底物,通過單因素試驗和正交試驗優化瞭底物的酶解條件.結果錶明：酶解條件的主次順序為底物質量濃度、酶質量濃度、酶解時間、酶解溫度和pH；最佳酶解條件為底物質量濃度0.25 g/L、酶質量濃度2 g/L、酶解時間36 h、酶解溫度45℃和pH為5.0,此時總還原糖含量最大為707.07 mg/g,糖含量顯著增加.酶解對于提高麥稈的糖化率是非常有必要的.
이희류산예처리적맥간위저물,통과단인소시험화정교시험우화료저물적매해조건.결과표명：매해조건적주차순서위저물질량농도、매질량농도、매해시간、매해온도화pH；최가매해조건위저물질량농도0.25 g/L、매질량농도2 g/L、매해시간36 h、매해온도45℃화pH위5.0,차시총환원당함량최대위707.07 mg/g,당함량현저증가.매해대우제고맥간적당화솔시비상유필요적.
Enzymolysis conditions were optimized by single factor experiment and orthogonal experiments using wheat straw which was pretreated by dilute sulphuric acid (H2SO4).The results showed that the primary and secondary factors of enzymolysis conditions were substrate concentration,enzyme concentration,enzymatic time, enzymatic temperature and pH.The optimal enzymolysis conditions were substrate concentration of 0.25 g/L,enzyme condition of 2 g/L,enzymatic time of 36 h,enzymatic temperature of 45 ℃ and pH of 5.0.The maximum of total reducing sugar concentration was 707.07 mg/g at the optimal enzymolysis conditions,sugar yield increased dramatically,indicating that enzymolysis is very necessary to improve the saccharification efficiency of wheat straw.
