CAJ | 학술논문

目的：研究蟾毒噻咛的抗肿瘤作用。方法：取3种肿瘤细胞株ECa109、HepG2、A549，每种细胞分为9组，分别加入0．125、0．250、0．500、1．000、2．000、4．000、8．000、16．000、32．000 mg/L的蟾毒噻咛处理48 h。采用MTT比色实验计算细胞增殖抑制率；采用流式细胞术检测HepG2细胞周期及凋亡。建立荷S180腹水瘤昆明小鼠模型，随机分为5组，每组20只，阴性对照组注射溶剂，阳性对照组每天给予20 mg/kg的5-FU，蟾毒噻咛低、中、高剂量组分别给予15、20和30 mg/kg的蟾毒噻咛，连续腹腔注射给药7 d。末次给药后每组随机抽取半数荷瘤小鼠，收集腹水，计算腹水抑制率和瘤细胞存活率；剩余小鼠统计生存时间，计算生命延长率。结果：随着药物质量浓度的增大，蟾毒噻咛对3种肿瘤细胞的抑制作用增强，其中对HepG2细胞的抑制作用最强（ F＝6．785、29．641和14.455，P＜0．05）；蟾毒噻咛能诱发HepG2细胞凋亡，将细胞周期阻滞在G2期。蟾毒噻咛还能提高荷瘤鼠腹水抑制率，抑制瘤细胞存活率，提高荷瘤鼠生命延长率（F＝412．321、900．735和1151．272，P＜0．05）。结论：蟾毒噻咛具有抗肿瘤作用。
목적：연구섬독새녕적항종류작용。방법：취3충종류세포주ECa109、HepG2、A549，매충세포분위9조，분별가입0．125、0．250、0．500、1．000、2．000、4．000、8．000、16．000、32．000 mg/L적섬독새녕처리48 h。채용MTT비색실험계산세포증식억제솔；채용류식세포술검측HepG2세포주기급조망。건립하S180복수류곤명소서모형，수궤분위5조，매조20지，음성대조조주사용제，양성대조조매천급여20 mg/kg적5-FU，섬독새녕저、중、고제량조분별급여15、20화30 mg/kg적섬독새녕，련속복강주사급약7 d。말차급약후매조수궤추취반수하류소서，수집복수，계산복수억제솔화류세포존활솔；잉여소서통계생존시간，계산생명연장솔。결과：수착약물질량농도적증대，섬독새녕대3충종류세포적억제작용증강，기중대HepG2세포적억제작용최강（ F＝6．785、29．641화14.455，P＜0．05）；섬독새녕능유발HepG2세포조망，장세포주기조체재G2기。섬독새녕환능제고하류서복수억제솔，억제류세포존활솔，제고하류서생명연장솔（F＝412．321、900．735화1151．272，P＜0．05）。결론：섬독새녕구유항종류작용。
Aim:To investigate the anti-tumor activity of bufothionine , which is an extract of toad skin .Methods:ECa109,HepG2,and A549 cells were used to assess the inhibitory effect of bufothionine .The cells were all allocated into 9 groups and treated with bufothionine at 0.125,0.250,0.500,1.000,2.000,4.000,8.000,16.000,and 32.000 μg/L.The inhibition effect of bufothionine was analyzed by MTT assay .The cell apoptosis and the cell cycle distribution ,by flow cy-tometry.Kunming mice bearing ascites tumor were allocated into 5 groups at random:control group,5-fluorouracil group[20 mg/(kg· d)],bufuthionine low-,middle-,and high-dose[30,20,15 mg/(kg· d)] groups.The treatments were given 7 times and the interval was 24 h.After the last treatment,a half mice were executed and the ascites were collected .Ascites inhibition rate and tumor cell persistence rate were calculated .The other half were used to observe the survival status .Re-sults:Bufothionine had dose-dependent inhibitive effects on the tumor cells especially on HepG 2(F=6.785,29.641,and 14.455,P<0.05).Bufothionine could induce cell apoptosis and arrest the cells at G 2 phase;bufothionine could inhibit the produce of ascites and the survival of tumor cells , and prolong the survival of mice bearing ascites tumor ( F=412 .321 , 900.735 and 1 151.272,P<0.05).Conclusion:Bufothionine has anti-tumor effects.