CAJ | 학술논문

目的：观察加味三甲散对被动皮肤致敏型大鼠外周血单核细胞CD4＋CD25＋Treg mRNA表达的影响。方法：60只成年SD大鼠随机分为正常对照组，模型对照组，氯雷他定组和加味三甲散高、中、低剂量组，后5组均制作被动皮肤致敏模型。造模后氯雷他定组按10 mL/kg剂量灌胃给予氯雷他定水溶液，加味三甲散高、中、低剂量组分别按38．0、19．0、9．5 g/kg剂量灌胃给予加味三甲散提取浓缩液，正常对照组和模型对照组灌胃给予等体积纯净水。每天1次，连续3周。实验结束时，收集各组大鼠血液，采用实时荧光定量PCR技术检测CD4＋CD25＋Treg mRNA的表达。结果：6组大鼠外周血单核细胞 CD4＋CD25＋Treg mRNA 的表达水平差异有统计学意义（ F ＝92.170，P＜0．001）；与正常对照组比较，模型对照组大鼠外周血单核细胞CD4＋CD25＋Treg mRNA的表达降低；与模型对照组比较，加味三甲散高、中、低剂量组大鼠外周血单核细胞CD4＋CD25＋Treg mRNA的表达升高（ P均＜0.05）。结论：加味三甲散可上调被动皮肤致敏型大鼠外周血单核细胞异常下降的CD4＋CD25＋Treg mRNA的表达水平，对慢性荨麻疹患者外周血单核细胞中CD4＋CD25＋Treg细胞功能的缺陷可能具有一定的干预作用。
목적：관찰가미삼갑산대피동피부치민형대서외주혈단핵세포CD4＋CD25＋Treg mRNA표체적영향。방법：60지성년SD대서수궤분위정상대조조，모형대조조，록뢰타정조화가미삼갑산고、중、저제량조，후5조균제작피동피부치민모형。조모후록뢰타정조안10 mL/kg제량관위급여록뢰타정수용액，가미삼갑산고、중、저제량조분별안38．0、19．0、9．5 g/kg제량관위급여가미삼갑산제취농축액，정상대조조화모형대조조관위급여등체적순정수。매천1차，련속3주。실험결속시，수집각조대서혈액，채용실시형광정량PCR기술검측CD4＋CD25＋Treg mRNA적표체。결과：6조대서외주혈단핵세포 CD4＋CD25＋Treg mRNA 적표체수평차이유통계학의의（ F ＝92.170，P＜0．001）；여정상대조조비교，모형대조조대서외주혈단핵세포CD4＋CD25＋Treg mRNA적표체강저；여모형대조조비교，가미삼갑산고、중、저제량조대서외주혈단핵세포CD4＋CD25＋Treg mRNA적표체승고（ P균＜0.05）。결론：가미삼갑산가상조피동피부치민형대서외주혈단핵세포이상하강적CD4＋CD25＋Treg mRNA적표체수평，대만성담마진환자외주혈단핵세포중CD4＋CD25＋Treg세포공능적결함가능구유일정적간예작용。
Aim:To study the effect of modified Sanjiasan on the expression of CD 4 +CD25 +Treg mRNA in peripheral blood monocytes of rats with passive skin sensitization .Methods:A total of 60 rats were allocated into 6 groups:normal con-trol group,model control group,loratadine group,Sanjiasan high-,middle-and low-dose groups.The latter 5 groups were made passive skin sensitization model .The rats in the loratadine group were given 10 mL/kg loratadine by gavage ,the rats in Sanjiasan high-,middle-and low-dose groups were given 38.0,19.0,and 9.5 g/kg Sanjiasan,and the rats in normal control group and model control group were given normal saline ,once a day,for 3 weeks.Blood samples were collected to de-tect the expression of CD4 +CD25 +Treg mRNA by real time-PCR.Results:There were significant differences in the expres-sion of CD4 +CD25 +Treg mRNA among the 6 groups(F=92.170,P<0.001);compared with normal control,the expres-sion of CD4+CD25 +Treg mRNA in model control group decreased;compared with model control group ,the expression of CD4 +CD25 +Treg mRNA in Sanjiasan high-,middle-and low-dose groups elevated(P<0.05).Conclusion:Modified San-jiasan can upregulate the expression of CD 4 +CD25 +Treg mRNA that decreases in peripheral blood monocytes of rats with passive skin sensitization .Modified Sanjiasan may have certain intervention effect on the patients with chronic urticaria .