CAJ | 학술논문

目的：观察羧甲基壳聚糖（CMCS）温敏凝胶对病变牙根面NIH3T3细胞附着和增殖的影响。方法：60颗患牙制成60个牙根片，分为5组。 A组：超声刮治＋EDTA处理3 min＋CMCS浸提液涂擦牙根表面5 min；B组：超声刮治＋EDTA处理3 min＋无菌生理盐水涂擦牙根表面5 min；C组：手动刮治＋EDTA处理3 min＋CMCS浸提液涂擦牙根表面5 min；D组：手动刮治＋EDTA处理3 min＋无菌生理盐水涂擦牙根表面5 min；E组（对照组）：EDTA处理3 min＋无菌生理盐水涂擦牙根表面5 min。将NIH3T3细胞与5组牙根片分别共培养16、24、72 h，采用细胞计数法和MTT法观察结果。结果：培养16 h时，A、B、C、D组细胞计数均高于对照组。培养24、72 h时，A、B、C、D组细胞增殖能力均高于对照组，且使用CMCS浸提液的A组及C组的细胞增殖能力高于使用无菌生理盐水的B组及D组（F＝31．813和41．579，P＜0．05）。结论：采用手动或超声进行牙周刮治及根面平整均有助于NIH3T3细胞的附着；联合使用CMCS温敏凝胶处理牙根表面可以促进NIH3T3细胞在牙根面的附着与增殖。
목적：관찰최갑기각취당（CMCS）온민응효대병변아근면NIH3T3세포부착화증식적영향。방법：60과환아제성60개아근편，분위5조。 A조：초성괄치＋EDTA처리3 min＋CMCS침제액도찰아근표면5 min；B조：초성괄치＋EDTA처리3 min＋무균생리염수도찰아근표면5 min；C조：수동괄치＋EDTA처리3 min＋CMCS침제액도찰아근표면5 min；D조：수동괄치＋EDTA처리3 min＋무균생리염수도찰아근표면5 min；E조（대조조）：EDTA처리3 min＋무균생리염수도찰아근표면5 min。장NIH3T3세포여5조아근편분별공배양16、24、72 h，채용세포계수법화MTT법관찰결과。결과：배양16 h시，A、B、C、D조세포계수균고우대조조。배양24、72 h시，A、B、C、D조세포증식능력균고우대조조，차사용CMCS침제액적A조급C조적세포증식능력고우사용무균생리염수적B조급D조（F＝31．813화41．579，P＜0．05）。결론：채용수동혹초성진행아주괄치급근면평정균유조우NIH3T3세포적부착；연합사용CMCS온민응효처리아근표면가이촉진NIH3T3세포재아근면적부착여증식。
Aim:To observe the effect of carboxymethyl chitosan ( CMCS) thermosensitive hydrogel on the attachment and proliferation of NIH3T3 cells on the root slices with periodontitis after scaling and root planning (SRP).Methods:Sixty teeth with periodontitis were used to prepare root slices and allocated into 5 groups randomly .Group A and group B were trea-ted by SRP using ultrasonator ,and group C and group D through manual treatment .After sterilizing the slices ,all of them were treated by EDTA for 3 min,then group A and group C were smeared with CMCS thermosensitive hydrogel ,and the other 3 groups were smeared by normal saline for 5 min.Then the NIH3T3 cells and the root slices were cocultured for 16,24,and 72 h,and the results were observed by cell counting method or MTT assay .Results:At 16 h,there were more cells in groups A,B, C,and D than those in control.At 24 and 72 h,the OD values of groups A,B,C,and D were higher than those in control;the OD values of groups A and C using CMCS thermosensitive hydrogel were higher than those in groups B and D using normal saline (F=31.813 and 41.579,P<0.05).Conclusion:Treating the teeth with SRP using ultrasonator or by hand both help the attach-ment of NIH3T3 cells.CMCS thermosensitive hydrogel could promote the adhesion and proliferation of NIH3T3 cells.