CAJ | 학술논문

对自中华鳖浅红色养殖水体中分离出的1株光合细菌进行菌体和菌落的形态观察、生理生化特征分析及16S rDNA序列相似性分析，将鉴定出的菌株用海藻酸钠法固化，在实验室规模开展对中华鳖养殖水体的处理试验。试验结果表明，菌落为深红色圆形菌落，菌体短杆状，革兰氏阴性，能运动，为兼性厌氧菌。鉴定该菌株为类球红细菌。对固化细菌的包埋材料-琼脂、海藻酸钠、聚乙烯醇＋海藻酸钠进行比较，其中3％海藻酸钠溶液与4％ C aC l2溶液交联，成球质量好，操作简便，被选作包埋材料。水处理结果显示，6 d时游离菌液组和固化细菌组的化学需氧量去除率分别为42．2％、65．1％，氨氮去除率分别为43．1％、62．1％，固化类球红细菌明显提高了水质处理效果。
대자중화별천홍색양식수체중분리출적1주광합세균진행균체화균락적형태관찰、생리생화특정분석급16S rDNA서렬상사성분석，장감정출적균주용해조산납법고화，재실험실규모개전대중화별양식수체적처리시험。시험결과표명，균락위심홍색원형균락，균체단간상，혁란씨음성，능운동，위겸성염양균。감정해균주위류구홍세균。대고화세균적포매재료-경지、해조산납、취을희순＋해조산납진행비교，기중3％해조산납용액여4％ C aC l2용액교련，성구질량호，조작간편，피선작포매재료。수처리결과현시，6 d시유리균액조화고화세균조적화학수양량거제솔분별위42．2％、65．1％，안담거제솔분별위43．1％、62．1％，고화류구홍세균명현제고료수질처리효과。
A strain of photosynthetic bacterium was isolated from the “red water” in Chinese soft shelled turtle Trionyx sinensis culture ponds ,and were identified and immobilized by morphological ,physiological and biochemical characteristics and 16S rDNA sequencing .It was found that the bacterium with dull red round and regular colonies was short rod-shaped ,gram-negative ,motile ,and cultative anaerobic ,and i-dentified as Rhodobacter sphaeroides .The immobilized material screening showed that good quality cross-linked microsphere was observed in 3% sodium alginate solution with 4% CaCl2 solution .The Chinese soft shelled turtle culture water treated by immobilized R .sphaeroides in a laboratory modulated experiment showed that 42 .2% of COD and 43 .1% of ammonia nitrogen were eliminated from the waters in free cells group and 65 .1% of COD ,and 62 .1% of ammonia nitrogen in the immobilized cells ,indicating that immo-bilization of the bacteria improves the treatment effect of aquaculture waste waters .The findings will pro-vide reference to the treatment of wastewater produced by Chinese soft shelled turtle or other cultured ani-mals .