中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
9期
1598-1600
,共3页
高效液相色谱法%维生素B12片%含量测定%含量均匀度
高效液相色譜法%維生素B12片%含量測定%含量均勻度
고효액상색보법%유생소B12편%함량측정%함량균균도
HPLC%Vitamin B12 tablets%Content determination%Uniformity
目的：建立HPLC方法测定维生素B12片的含量及含量均匀度。方法：色谱柱为Agilet Eclipse XDB-C18(250 mm ×4.6 mm,5μm),流动相为0.02 mol·L-1磷酸氢二钠溶液-甲醇(74∶26),流速为1.0 ml·min-1,检测波长为225 nm,柱温为30℃,进样量为20μl。结果：维生素B12在1.67~41.72μg·ml-1浓度范围内与峰面积线性关系良好(r=1.0000)。平均回收率为99.9%(RSD=0.40%,n=9)。结论：本方法结果准确可靠,重复性好,可作为维生素B12片的含量及含量均匀度测定方法。
目的：建立HPLC方法測定維生素B12片的含量及含量均勻度。方法：色譜柱為Agilet Eclipse XDB-C18(250 mm ×4.6 mm,5μm),流動相為0.02 mol·L-1燐痠氫二鈉溶液-甲醇(74∶26),流速為1.0 ml·min-1,檢測波長為225 nm,柱溫為30℃,進樣量為20μl。結果：維生素B12在1.67~41.72μg·ml-1濃度範圍內與峰麵積線性關繫良好(r=1.0000)。平均迴收率為99.9%(RSD=0.40%,n=9)。結論：本方法結果準確可靠,重複性好,可作為維生素B12片的含量及含量均勻度測定方法。
목적：건립HPLC방법측정유생소B12편적함량급함량균균도。방법：색보주위Agilet Eclipse XDB-C18(250 mm ×4.6 mm,5μm),류동상위0.02 mol·L-1린산경이납용액-갑순(74∶26),류속위1.0 ml·min-1,검측파장위225 nm,주온위30℃,진양량위20μl。결과：유생소B12재1.67~41.72μg·ml-1농도범위내여봉면적선성관계량호(r=1.0000)。평균회수솔위99.9%(RSD=0.40%,n=9)。결론：본방법결과준학가고,중복성호,가작위유생소B12편적함량급함량균균도측정방법。
Objective:To establish an HPLC method for the determination of content and content uniformity of vitamin B12 tablets. Method:The HPLC method was conducted on a C18 column. The mobile phase was 0. 02 mol·L-1 disodium hydrogen phosphate solu-tion-methanol(74∶26). The flow rate was 1. 0 ml·min-1 and the detection wavelength was 225nm. The column temperature was 30℃and the injection volume was 20 μl. Result: The linearity of vitamin B12 was good within the range of 1. 67-41. 72 μg·ml-1 ( r=1. 000 0). The average recovery was 99. 9%(RSD=0. 40%,n=9). Conclusion:The method is accurate,reliable and reproducible, and can be used in the determination of content and content uniformity of vitamin B12 tablets.
