CAJ | 학술논문

目的：建立HPLC法同时测定氯甲醑中氯霉素及甲硝唑的含量。方法：采用HPLC法,色谱柱为Nov-pak C18(150 mm×4.6 mm,5μm),流动相为甲醇∶水(75∶25),流速为1.0 ml·min-1,检测波长为297 nm,柱温：30℃,进样量20μl。结果：甲硝唑和氯霉素线性范围分别为5~80μg·ml-1(r =0.9997)和50~800μg·ml-1(r =0.9998)；平均回收率分别为100.83%(RSD=1.82%,n=6)和100.20%(RSD=0.55%,n=6)。结论：本法快速方便、精密度高、重复性和稳定性好,可用于同时测定氯甲醑中氯霉素和甲硝唑的含量。
목적：건립HPLC법동시측정록갑서중록매소급갑초서적함량。방법：채용HPLC법,색보주위Nov-pak C18(150 mm×4.6 mm,5μm),류동상위갑순∶수(75∶25),류속위1.0 ml·min-1,검측파장위297 nm,주온：30℃,진양량20μl。결과：갑초서화록매소선성범위분별위5~80μg·ml-1(r =0.9997)화50~800μg·ml-1(r =0.9998)；평균회수솔분별위100.83%(RSD=1.82%,n=6)화100.20%(RSD=0.55%,n=6)。결론：본법쾌속방편、정밀도고、중복성화은정성호,가용우동시측정록갑서중록매소화갑초서적함량。
Objective: To establish an HPLC method for the simultaneous determination of chloromycetin and metronidazole in chlortalidone and metronidazole spirits. Method:The HPLC method was performed on an NOV-pak C18 (150 mm × 4. 6 mm, 5 μm) column and the mobile phase consisted of methanol and water (75∶25). The flow rate was 1. 0 ml·ml-1, the detection wavelength was 297nm, the temperature of column was 30℃ and the sample size was 20 μl. Result: The calibration curve of metronidazole was linear within the range of 5-80μg·ml-1(r=0. 999 7), and the average recovery was 100. 83% with RSD of 1. 82%(n=6). The cal-ibration curve of chlortalidone was linear within the range of 50-800μg·ml-1(r=0.999 7), and the average recovery was 100.2%with RSD of 0. 55%(n=6). Conclusion: The method is simple, rapid, accurate and reproducible, and can be used in the quality control of chlortalidone and metronidazole spirits.