中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
9期
1594-1595,1596
,共3页
郭迎霞%何栋梁%宋静莉%栗娟
郭迎霞%何棟樑%宋靜莉%慄娟
곽영하%하동량%송정리%률연
杯苋甾酮%高效液相色谱法%肾石消胶囊
杯莧甾酮%高效液相色譜法%腎石消膠囊
배현치동%고효액상색보법%신석소효낭
Cyasterone%HPLC%Shenshixiao capsules
目的：建立肾石消胶囊中杯苋甾酮的含量测定方法。方法：采用HPLC法,色谱柱为Agilent C18(250 mm ×4.6 mm,5μm),以甲醇-水为流动相梯度洗脱,检测波长：243 nm,流速：1.0 ml·min-1,柱温：室温。结果：杯苋甾酮进样量在0.025~0.247μg的浓度范围内线性关系良好,r=0.9996,平均回收率为99.4%,RSD为0.76%(n=9)。结论：该方法快速、简单、结果准确、可用于该制剂质量控制的一项重要指标。
目的：建立腎石消膠囊中杯莧甾酮的含量測定方法。方法：採用HPLC法,色譜柱為Agilent C18(250 mm ×4.6 mm,5μm),以甲醇-水為流動相梯度洗脫,檢測波長：243 nm,流速：1.0 ml·min-1,柱溫：室溫。結果：杯莧甾酮進樣量在0.025~0.247μg的濃度範圍內線性關繫良好,r=0.9996,平均迴收率為99.4%,RSD為0.76%(n=9)。結論：該方法快速、簡單、結果準確、可用于該製劑質量控製的一項重要指標。
목적：건립신석소효낭중배현치동적함량측정방법。방법：채용HPLC법,색보주위Agilent C18(250 mm ×4.6 mm,5μm),이갑순-수위류동상제도세탈,검측파장：243 nm,류속：1.0 ml·min-1,주온：실온。결과：배현치동진양량재0.025~0.247μg적농도범위내선성관계량호,r=0.9996,평균회수솔위99.4%,RSD위0.76%(n=9)。결론：해방법쾌속、간단、결과준학、가용우해제제질량공제적일항중요지표。
Objective: To establish a method for the determination of cyasterone in Shenshixiao capsules. Methods: Using an HPLC method, the column was an Agilent C18 (250 mm × 4. 6 mm,5 μm) column, the mobile phase was methanol-water with gradient elution. The detection wavelength was 243nm, the flow rate was 1. 0 ml·min-1 ,and the temperature of column was room temperature. Results:The linear relationship of cyasterone was good within the concentration range of 0. 025-0. 247 μg(r=0. 999 6), the average recovery was 99. 4%, and RSD was 0. 76%. Conclusion:The method is rapid, simple and accurate, and can be used in the quality control of the preparation.
