中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
9期
1584-1585
,共2页
金线莲%高效液相色谱法%芦丁%含量
金線蓮%高效液相色譜法%蘆丁%含量
금선련%고효액상색보법%호정%함량
Anoectochilus roxburghii%HPLC%Rutin%Content
目的：建立金线莲中芦丁的含量测定方法。方法：采用高效液相色谱法。选用Lanbo-Kromasil-C18色谱柱(250 mm ×
目的：建立金線蓮中蘆丁的含量測定方法。方法：採用高效液相色譜法。選用Lanbo-Kromasil-C18色譜柱(250 mm ×
목적：건립금선련중호정적함량측정방법。방법：채용고효액상색보법。선용Lanbo-Kromasil-C18색보주(250 mm ×
Objective:To establish a method for the determination of rutin in Anoectochili roxburghii. Methods:The detection was carried out by HPLC. A Lanbo-Kromasil C18 column(250 mm × 4. 6 mm,5 μm) was used with methanol-0. 2% phosphoric acid solu-tion (40∶60) as the mobile phase;the detection wavelength was 257nm, the flow rate was 1. 0 ml·min-1 and the column temperature was 30℃. Results:Rutin showed a good linear relationship within the range of 0. 021-0. 433 μg(r=0. 999 6). The average recovery was 96. 65%(RSD=1. 67%, n=9). Conclusion:The method is simple, rapid, specific and reproducible.
