CAJ | 학술논문

目的：建立玉女煎颗粒剂的质量标准。方法：采用TLC对处方中熟地、知母、牛膝进行鉴别；用HPLC测定地黄中毛蕊花糖苷的含量。色谱柱为SunfireTM C18(250 mm ×4.6 mm,5μm)；流动相为乙腈-0.1%醋酸溶液(16∶84),柱温：30℃,流速：1.0 ml·min-1,检测波长334 nm,进样量：20μl。结果：在TLC色谱中检出熟地的特征成份毛蕊花糖苷、知母的特征成份菝葜皂苷元、牛膝特征成份杯苋甾酮,斑点清晰,分离度好；毛蕊花糖苷在0.163-0.612μg与峰面积呈良好的线性关系,r=0.9999,平均回收率为101.3%,RSD为2.7%(n=6)。结论：该方法简便、可靠、准确,适用于该剂型的质量控制。
목적：건립옥녀전과립제적질량표준。방법：채용TLC대처방중숙지、지모、우슬진행감별；용HPLC측정지황중모예화당감적함량。색보주위SunfireTM C18(250 mm ×4.6 mm,5μm)；류동상위을정-0.1%작산용액(16∶84),주온：30℃,류속：1.0 ml·min-1,검측파장334 nm,진양량：20μl。결과：재TLC색보중검출숙지적특정성빈모예화당감、지모적특정성빈발계조감원、우슬특정성빈배현치동,반점청석,분리도호；모예화당감재0.163-0.612μg여봉면적정량호적선성관계,r=0.9999,평균회수솔위101.3%,RSD위2.7%(n=6)。결론：해방법간편、가고、준학,괄용우해제형적질량공제。
Objective:To establish the quality standard for Yunüjian granules. Methods:Rehmannia glutinosa, rhizoma anemar-rhenae and achyranthes in the granules were identified by TLC. The HPLC method was adopted. A SunfireTM C18 column (250 mm × 4. 6 mm, 5 μm) was used with the mobile phase consisting of acetonitrile-0. 1% acetic acid solution(16∶84) at the column tempera-ture of 30 ℃. The flow rate was 1. 0 ml·min-1 ,the detection wavelength was 334nm, and the imjection volume was 10 μl. Results:The TLC spots of verbascoside, and sarsasapogenin in rhizoma anemarrhenae and cyasterone in achyranthes were quite clear with good seperation. The linear range of verbascoside was within the range of 0.163-0.612 μg(r=0.999 9), and the average recovery was 101. 3%(RSD=2. 7%,n=6). Conclusion:The method is simple, reliable and accurate, and can be applied in the quality control of Ynnüjian granules.