CAJ | 학술논문

目的：建立血浆中瑞芬太尼高效液相色谱测定方法，为临床合理用药提供依据。方法血浆样品用1-氯丁烷进行萃取，上清液提取至0.01mol/L盐酸溶液中。分析柱为spherisorb-CN，流动相为0.02mol/L的NaH2 PO4水溶液-乙腈（70：30），内含三乙胺0.01%，流速1.5mL/min，柱温为室温，紫外检测波长为210nm，进样量为200μL。选择择期行胸科手术患者8例，靶控输注瑞芬太尼浓度（ Cp）6μg/L复合异丙酚2.5mg/kg，分别在瑞芬太尼靶控输注前及靶控输注后5、10、20、30、60、90min时抽取桡动脉血，用高效液相色谱法测定瑞芬太尼血药浓度（Cm）。结果人血浆中瑞芬太尼检测浓度在2.5~300μg/L范围内线性关系良好（r=0.9991）。日内日间精密度均在10%以内，提取回收率大于78.57%，方法回收率在96.03%~104.67%。在靶控输注5~20min时，瑞芬太尼测定浓度低于靶控输注浓度（ p＜0.05），30~90min两者差异无统计学意义（ p＞0.05）。结论该方法操作简便，分离度好，其专属性、准确度、精密度、线性范围及稳定性均适合实际样品的检测。
목적：건립혈장중서분태니고효액상색보측정방법，위림상합리용약제공의거。방법혈장양품용1-록정완진행췌취，상청액제취지0.01mol/L염산용액중。분석주위spherisorb-CN，류동상위0.02mol/L적NaH2 PO4수용액-을정（70：30），내함삼을알0.01%，류속1.5mL/min，주온위실온，자외검측파장위210nm，진양량위200μL。선택택기행흉과수술환자8례，파공수주서분태니농도（ Cp）6μg/L복합이병분2.5mg/kg，분별재서분태니파공수주전급파공수주후5、10、20、30、60、90min시추취뇨동맥혈，용고효액상색보법측정서분태니혈약농도（Cm）。결과인혈장중서분태니검측농도재2.5~300μg/L범위내선성관계량호（r=0.9991）。일내일간정밀도균재10%이내，제취회수솔대우78.57%，방법회수솔재96.03%~104.67%。재파공수주5~20min시，서분태니측정농도저우파공수주농도（ p＜0.05），30~90min량자차이무통계학의의（ p＞0.05）。결론해방법조작간편，분리도호，기전속성、준학도、정밀도、선성범위급은정성균괄합실제양품적검측。
OcjectiVe High performance liquid chromarography ( HPLC ) method was established for determination of remifentanil in plasma. The study will provide academic foundation for the development and clinical use of remifentanil. Methods The samples eXtracted with 1-chlorobutane and supernatant was back eXtracted into 0. 01mol/L HCl again. The separation was performed on a spherisorb-CN column with a mobile phase of 0. 02mol/L-1NaH2 PO4-acetonitrile( 70: 30 ) including 0. 01%triethylamine at a flow rate of 1. 5mL/min. The column temperature was room temperature,the detection-wavelength was 210nm and 200μL of solution was injected into HPLC. Eight patients undergoing thoracic surgery were assigned to receive remifentanil target controlled infusion( TCI )( 6μg/L ) and propofol intravenous injection(2. 5mg/kg). Arterial blood samples were taken before and after TCI remifentanil 5, 10,20,30,60,90 min for measuring of blood concentration of remifentanil by HPLC. Results A linearity was obtained from 2 . 5μg/L to 300μg/L of remifentanil in plasma with a good correlation( r=0 . 999 1 ). The between-day and within-day RSD% were less than 10%. The eXtraction recoveries were more than 78 . 57%,and the method recoveries were between 96 . 03% and 104 . 67%. The determination of the concentration was lower than target plasma concentration at TCI 5 min to 20 min( p<0 . 05 )and there was no significant difference from 30 min to 90 min( p >0 . 05 ). Conclusion This HPLC method is simple,sensitive and accurate,and it is suitable for routine determination of remifentanil levels in human plasma.