河北医科大学学报
河北醫科大學學報
하북의과대학학보
JOURNAL OF HEBEI MEDICAL UNIVERSITY
2014年
9期
997-999
,共3页
侯永革%王保芝%王金立%王俊峰%崔芳%孟丽
侯永革%王保芝%王金立%王俊峰%崔芳%孟麗
후영혁%왕보지%왕금립%왕준봉%최방%맹려
脉络丛%侧脑室%细胞结构%大鼠
脈絡叢%側腦室%細胞結構%大鼠
맥락총%측뇌실%세포결구%대서
choroid pleXus%lateral ventricles%cellular structures%rats
目的:探讨大鼠侧脑室脉络丛及丛上细胞的结构特点。方法选用成年雄性SD大鼠5只,在深度麻醉下通过主动脉灌注醛类固定液,取侧脑室脉络丛组织制备样品,进行扫描电镜和透射电镜观察。结果扫描电镜观察显示,侧脑室脉络丛表面由平行方式形成的皱襞或脊组成,上皮细胞游离面可见大量微绒毛;脉络丛表面可见许多丛上细胞,其胞体带有伪足样突起。透射电镜观察显示,脉络丛上皮细胞下为有孔型毛细血管,上皮细胞之间为缝隙连接;脉络丛上皮细胞内有丰富的线粒体和分泌囊泡;丛上细胞的细胞体和伪足状突起内,可见次级溶酶体或吞噬体。结论大鼠侧脑室脉络丛及丛上细胞的细微和超微结构特点,为深入研究其生理和病理意义,提供了形态学基础。
目的:探討大鼠側腦室脈絡叢及叢上細胞的結構特點。方法選用成年雄性SD大鼠5隻,在深度痳醉下通過主動脈灌註醛類固定液,取側腦室脈絡叢組織製備樣品,進行掃描電鏡和透射電鏡觀察。結果掃描電鏡觀察顯示,側腦室脈絡叢錶麵由平行方式形成的皺襞或脊組成,上皮細胞遊離麵可見大量微絨毛;脈絡叢錶麵可見許多叢上細胞,其胞體帶有偽足樣突起。透射電鏡觀察顯示,脈絡叢上皮細胞下為有孔型毛細血管,上皮細胞之間為縫隙連接;脈絡叢上皮細胞內有豐富的線粒體和分泌囊泡;叢上細胞的細胞體和偽足狀突起內,可見次級溶酶體或吞噬體。結論大鼠側腦室脈絡叢及叢上細胞的細微和超微結構特點,為深入研究其生理和病理意義,提供瞭形態學基礎。
목적:탐토대서측뇌실맥락총급총상세포적결구특점。방법선용성년웅성SD대서5지,재심도마취하통과주동맥관주철류고정액,취측뇌실맥락총조직제비양품,진행소묘전경화투사전경관찰。결과소묘전경관찰현시,측뇌실맥락총표면유평행방식형성적추벽혹척조성,상피세포유리면가견대량미융모;맥락총표면가견허다총상세포,기포체대유위족양돌기。투사전경관찰현시,맥락총상피세포하위유공형모세혈관,상피세포지간위봉극련접;맥락총상피세포내유봉부적선립체화분비낭포;총상세포적세포체화위족상돌기내,가견차급용매체혹탄서체。결론대서측뇌실맥락총급총상세포적세미화초미결구특점,위심입연구기생리화병리의의,제공료형태학기출。
OcjectiVe The study was to eXamine structural features of the choroid pleXus and epipleXus cells of the rat lateral ventricles. Methods Five adult male SD rats were anesthetized deeply with 6% chloral hydrate intra-peritoneal cavity and aortic perfusion with 3% polyformaldehyde and 1%glutaraldehyde,the samples of the choroid pleXus were collected and observed with scanning electron microscope( SEM)and transmission electron microscope( TEM). Results SEM showed the surface of the choroid pleXus of the rat lateral ventricles was constituted by folds or crests in a kind of parallel way,the free surface of the cells presented microvilli. There were pseudopodial projections on the surface of epipleXus cells. TEM showed that fenestrated capillaries can be seen in choroid pleXus subepithelial cells, gap junctions can be observed between epithelial cells,a lot of mitochondria and endocytosis bubbles can be seen in cytoplasm of epithelial cells. There were pseudopodial projections on the surface of epipleXus cells. Secondary lysosome can be seen in epipleXus cells. Conclusion The study showed that the fine and ultra-structural features of the choroid pleXus and epipleXus cells of the rat lateral ventricles may provide a morphological basis for further research of the physiological and pathological significance.