食用菌学报
食用菌學報
식용균학보
ACTA EDULIS FUNGI
2014年
2期
72-77
,共6页
药用拟层孔菌%化学成分%抗肿瘤
藥用擬層孔菌%化學成分%抗腫瘤
약용의층공균%화학성분%항종류
Fomitopsis officinalis%chemical composition%anti-proliferative activity
通过硅胶柱层析法、Sephadex LH-20凝胶柱层析法和重结晶法,分离纯化药用拟层孔菌(Fomitopsis officinalis)子实体中的化学成分,依据化合物的物理性质,运用核磁共振谱(nuclear magnetic resonance, NMR)、质谱(mass spectrometry,MS)等波谱法分析鉴定化合物结构,并选用得率较高的4种化合物进行体外抗肿瘤实验。共分离出8个化合物,为4,6,8(14),22(23)-四烯-3-酮-麦角甾烷、麦角甾-7,22-二稀-3β-醇、3-酮基-去氢硫色多孔菌酸、去氢齿孔酸、阿里红酸A、去氢齿孔酮酸、阿里红酸 C、阿里红酸 B。其中化合物4,6,8(14),22(23)-四烯-3-酮-麦角甾烷为首次从药用拟层孔菌子实体中分离得到。阿里红酸A、阿里红酸 C、3-酮基-去氢硫色多孔菌酸抑制人乳腺癌细胞MCF-7活性较好,阿里红酸A、阿里红酸 C 抑制人肝癌细胞 SMMC-7721活性较好。
通過硅膠柱層析法、Sephadex LH-20凝膠柱層析法和重結晶法,分離純化藥用擬層孔菌(Fomitopsis officinalis)子實體中的化學成分,依據化閤物的物理性質,運用覈磁共振譜(nuclear magnetic resonance, NMR)、質譜(mass spectrometry,MS)等波譜法分析鑒定化閤物結構,併選用得率較高的4種化閤物進行體外抗腫瘤實驗。共分離齣8箇化閤物,為4,6,8(14),22(23)-四烯-3-酮-麥角甾烷、麥角甾-7,22-二稀-3β-醇、3-酮基-去氫硫色多孔菌痠、去氫齒孔痠、阿裏紅痠A、去氫齒孔酮痠、阿裏紅痠 C、阿裏紅痠 B。其中化閤物4,6,8(14),22(23)-四烯-3-酮-麥角甾烷為首次從藥用擬層孔菌子實體中分離得到。阿裏紅痠A、阿裏紅痠 C、3-酮基-去氫硫色多孔菌痠抑製人乳腺癌細胞MCF-7活性較好,阿裏紅痠A、阿裏紅痠 C 抑製人肝癌細胞 SMMC-7721活性較好。
통과규효주층석법、Sephadex LH-20응효주층석법화중결정법,분리순화약용의층공균(Fomitopsis officinalis)자실체중적화학성분,의거화합물적물이성질,운용핵자공진보(nuclear magnetic resonance, NMR)、질보(mass spectrometry,MS)등파보법분석감정화합물결구,병선용득솔교고적4충화합물진행체외항종류실험。공분리출8개화합물,위4,6,8(14),22(23)-사희-3-동-맥각치완、맥각치-7,22-이희-3β-순、3-동기-거경류색다공균산、거경치공산、아리홍산A、거경치공동산、아리홍산 C、아리홍산 B。기중화합물4,6,8(14),22(23)-사희-3-동-맥각치완위수차종약용의층공균자실체중분리득도。아리홍산A、아리홍산 C、3-동기-거경류색다공균산억제인유선암세포MCF-7활성교호,아리홍산A、아리홍산 C 억제인간암세포 SMMC-7721활성교호。
Eight compounds,isolated and purified from Fomitopsis officinalis fruit bodies by silica gel column chromatography,Sephadex LH-20 column chromatography and recrystallization,were identified by NMR and mass spectrometry as 4,6,8 (14),22 (23)-tetraen-3-one-ergos-tane,ergosta-7,22,dien-3β-ol,3-keto-dehydrosulfurenic acid,dehydroeburicoic acid,dehydroeburiconic acid,and fomefficinic acids A,B and C. 4,6,8 (14),22 (23)-tetraen-3-one-ergostane has been isolated from F.officinalis fruit bodies for the first time.Fomefficinic acids A and C,3-keto-dehydrosulfurenic acid and dehydroeburicoic acid were obtained in high yield and tested for anti-proliferative activity against two human cancer cell lines.Fomefficinic acids A and C,and 3-keto-dehydrosulfurenic acid,inhibited the growth of MCF-7 human breast cancer cells,while fomefficinic acids A and C inhibited the growth of SMMC-7721 human liver cancer cells.
