CAJ | 학술논문

以醇提物的黄酮含量为指标,通过正交试验优化了鲍姆木层孔菌(Phellinus baumii)米饭固体发酵条件,结果表明用直径5.5 cm,高10 cm的培养瓶装50 g大米,2.5%葡萄糖,0.3% KH2 PO4,0.15% MgSO4·7H2 O,按料液比1∶1.2(w/v)加蒸馏水,灭菌接种后26℃培养27 d,发酵产物用乙醇提取得到醇提物的黄酮含量达39.8%。另外,比较了4种提取方法获得的醇提物的得率和对H2 O2超氧阴离子和DPPH自由基的清除率,以评价其抗氧化活性,结果表明,烘干后乙醇冷浸提取法得到的醇提物得率最高,抗氧化活性最好。
이순제물적황동함량위지표,통과정교시험우화료포모목층공균(Phellinus baumii)미반고체발효조건,결과표명용직경5.5 cm,고10 cm적배양병장50 g대미,2.5%포도당,0.3% KH2 PO4,0.15% MgSO4·7H2 O,안료액비1∶1.2(w/v)가증류수,멸균접충후26℃배양27 d,발효산물용을순제취득도순제물적황동함량체39.8%。령외,비교료4충제취방법획득적순제물적득솔화대H2 O2초양음리자화DPPH자유기적청제솔,이평개기항양화활성,결과표명,홍간후을순랭침제취법득도적순제물득솔최고,항양화활성최호。
Orthogonal testing was used to optimize the conditions for flavone production during the solid-state cultivation of Phellinus baumii on a rice-based medium.Highest flavone levels (39.8%)in ethanolic extracts of cultures were recorded when cultivation was carried out using culture bottles (5.5 cm diameter,10 cm height)containing 50 g rice,2.5% glucose,0.3% KH2 PO3 ,0.15% MgSO4·7H2 O,sterilized at 121 ℃ for 2 hours,inoculated with three blocks (1 cm2 )of P.baumii inoculum grown on potato dextrose agar,and incubated at 26 ℃ for 25-30 days.The antioxidant activities of P.baumii extracts obtained by four different extraction methods were compared by measuring H2 O2 ,superoxide anion and DPPH free radical scavenging rates.Highest antioxidant activity was recorded when the solid-state culture was dried in an oven at 30 ℃, then immersed in 10 vols (w/v)of 80% ethanol for 24 h.After filtration,the solid residue was retained,the procedure repeated,and the combined filtrates were evaporated to dryness under vacuum using a rotary evaporator.