CAJ | 학술논문

探讨在20 L搅拌式发酵罐中,转速和通气量对桑黄菌丝生物量、胞外多糖产率、溶氧以及菌丝形态的影响。结果表明,实验范围内,转速为150 r/min时,桑黄菌丝体生物量最大(12.65 g/L),胞外多糖得率最高(2.99 g/L),相对溶氧下降最快,菌球小球状、较紧密、丝状体比例小；通气量为1∶0.65 vvm,桑黄菌丝体生物量最大(12.69 g/L),胞外多糖得率最高(3.00 g/L),相对溶氧下降最快,菌球大小均匀、紧密、生长良好。
탐토재20 L교반식발효관중,전속화통기량대상황균사생물량、포외다당산솔、용양이급균사형태적영향。결과표명,실험범위내,전속위150 r/min시,상황균사체생물량최대(12.65 g/L),포외다당득솔최고(2.99 g/L),상대용양하강최쾌,균구소구상、교긴밀、사상체비례소；통기량위1∶0.65 vvm,상황균사체생물량최대(12.69 g/L),포외다당득솔최고(3.00 g/L),상대용양하강최쾌,균구대소균균、긴밀、생장량호。
The effects of impellor speed and aeration rate on mycelial biomass and extracellular polysaccharide production,reducing sugar consumption and dissolved oxygen levels during growth of Phellinus baumii in a 20-L fermentor were determined.Highest biomass (12.65 g/L)and extracellular polysaccharide(2.99 g/L) yields,and the most rapid decrease in dissolved oxygen levels,were recorded when the impellor speed was set at 150 r/min.Mycelial pellets were globose and compact,and the proportion of hyphal filaments was low. Highest yields of mycelial biomass (12.69 g/L)and extracellular polysaccharide(3.0 g/L),and the most rapid decrease in dissolved oxygen levels,were recorded when the aeration rate was set at 1∶0.65 vvm (air volume/culture volume/min).At this setting,the fungal mycelium grew well and formed compact pellets of uniform size.