中医临床研究
中醫臨床研究
중의림상연구
CLINICAL JOURNAL OF CHINESE MEDICINE
2014年
22期
16-17
,共2页
牛蒡子苷元%肝癌%缝隙连接蛋白
牛蒡子苷元%肝癌%縫隙連接蛋白
우방자감원%간암%봉극련접단백
Niubangzi aglycone%Liver cancer%Connexin
目的：观察牛蒡子苷元在体外对人肝癌细胞HepG2缝隙连接蛋白（Cx）表达的影响。方法：采用Western blot 技术检测牛蒡子苷元对人肝癌细胞HepG2缝隙连接蛋白（Cx26/32/43）的表达。结果：Western blot 检测，用国产多克隆Cx一抗和进口单克隆Cx一抗的实验均显示：牛蒡子苷元等能明显提高人肝癌细胞HepG2Cx32的表达，且有时间、浓度依赖性，氧化剂H2O2能抑制牛蒡子苷元此作用；但牛蒡子苷元对HepG2的Cx43表达影响24h不明显，48h能明显提高Cx43表达。结论：牛蒡子苷元能提高人肝癌细胞Cx表达，增强细胞间缝隙连接功能。
目的：觀察牛蒡子苷元在體外對人肝癌細胞HepG2縫隙連接蛋白（Cx）錶達的影響。方法：採用Western blot 技術檢測牛蒡子苷元對人肝癌細胞HepG2縫隙連接蛋白（Cx26/32/43）的錶達。結果：Western blot 檢測，用國產多剋隆Cx一抗和進口單剋隆Cx一抗的實驗均顯示：牛蒡子苷元等能明顯提高人肝癌細胞HepG2Cx32的錶達，且有時間、濃度依賴性，氧化劑H2O2能抑製牛蒡子苷元此作用；但牛蒡子苷元對HepG2的Cx43錶達影響24h不明顯，48h能明顯提高Cx43錶達。結論：牛蒡子苷元能提高人肝癌細胞Cx錶達，增彊細胞間縫隙連接功能。
목적：관찰우방자감원재체외대인간암세포HepG2봉극련접단백（Cx）표체적영향。방법：채용Western blot 기술검측우방자감원대인간암세포HepG2봉극련접단백（Cx26/32/43）적표체。결과：Western blot 검측，용국산다극륭Cx일항화진구단극륭Cx일항적실험균현시：우방자감원등능명현제고인간암세포HepG2Cx32적표체，차유시간、농도의뢰성，양화제H2O2능억제우방자감원차작용；단우방자감원대HepG2적Cx43표체영향24h불명현，48h능명현제고Cx43표체。결론：우방자감원능제고인간암세포Cx표체，증강세포간봉극련접공능。
Objective: To observe intervention of Niubangzi aglycone on expression of HepG2Cx. Methods: Intervention of Niubangzi aglycone on expression of HepG2Cx26/32/43 was tested by Western blot. Results:Experiment of domestic polyclonal anti-Cx antibody and imported monoclonal anti-Cx antibody appeared that Niubangzi aglycone could obviously increase expression of HepG2Cx32 with time and concentration-dependent, oxidant H2O2 could inhibit the effect of Niubangzi aglycone; Niubangzi aglycone could not obviously affect expression of HepG2Cx43 within 24h, Niubangzi aglycone markedly improved expression of HepG2Cx43 within 48h with concentration-dependent. Conclusion:Niubangzi aglycone improved HepG2Cx expression and GJIC function.
