中国当代医药
中國噹代醫藥
중국당대의약
PERSON
2014年
24期
4-6,12
,共4页
何婷玉%杜玉文%陈肖楠%赵国强
何婷玉%杜玉文%陳肖楠%趙國彊
하정옥%두옥문%진초남%조국강
聚合酶β%siRNA%食管癌%放疗敏感性
聚閤酶β%siRNA%食管癌%放療敏感性
취합매β%siRNA%식관암%방료민감성
Polymeraseβ%siRNA%Esophageal carcinoma%Radiotherapeutic sensitivity
目的探讨siRNA沉默DNA聚合酶β(polβ)基因表达对食管癌EC9706细胞放疗敏感性的影响。方法利用脂质体将siRNA转染至EC9706细胞,采用实时荧光定量PCR检测3组不同转染的细胞中polβ mRNA的表达变化;不同剂量(0、2、4、6、8 Gy)照射3组细胞,CCK8方法检测各组细胞的存活率;用3 Gy照射,流式细胞术检测每组细胞的凋亡率。结果转染沉默polβ组polβ基因表达水平显著降低(P<0.01);CCK8检测结果显示,随着照射剂量的增加,细胞存活率呈下降趋势,其中沉默polβ组细胞存活率显著低于两个对照(P<0.05);沉默polβ组细胞凋亡率显著高于无关siRNA组或空白对照组(P<0.01),照射后沉默polβ组细胞的凋亡率增加量显著高于无关siRNA组和空白对照组(P<0.01)。结论沉默食管癌细胞polβ基因表达可增加其对放疗的敏感性。
目的探討siRNA沉默DNA聚閤酶β(polβ)基因錶達對食管癌EC9706細胞放療敏感性的影響。方法利用脂質體將siRNA轉染至EC9706細胞,採用實時熒光定量PCR檢測3組不同轉染的細胞中polβ mRNA的錶達變化;不同劑量(0、2、4、6、8 Gy)照射3組細胞,CCK8方法檢測各組細胞的存活率;用3 Gy照射,流式細胞術檢測每組細胞的凋亡率。結果轉染沉默polβ組polβ基因錶達水平顯著降低(P<0.01);CCK8檢測結果顯示,隨著照射劑量的增加,細胞存活率呈下降趨勢,其中沉默polβ組細胞存活率顯著低于兩箇對照(P<0.05);沉默polβ組細胞凋亡率顯著高于無關siRNA組或空白對照組(P<0.01),照射後沉默polβ組細胞的凋亡率增加量顯著高于無關siRNA組和空白對照組(P<0.01)。結論沉默食管癌細胞polβ基因錶達可增加其對放療的敏感性。
목적탐토siRNA침묵DNA취합매β(polβ)기인표체대식관암EC9706세포방료민감성적영향。방법이용지질체장siRNA전염지EC9706세포,채용실시형광정량PCR검측3조불동전염적세포중polβ mRNA적표체변화;불동제량(0、2、4、6、8 Gy)조사3조세포,CCK8방법검측각조세포적존활솔;용3 Gy조사,류식세포술검측매조세포적조망솔。결과전염침묵polβ조polβ기인표체수평현저강저(P<0.01);CCK8검측결과현시,수착조사제량적증가,세포존활솔정하강추세,기중침묵polβ조세포존활솔현저저우량개대조(P<0.05);침묵polβ조세포조망솔현저고우무관siRNA조혹공백대조조(P<0.01),조사후침묵polβ조세포적조망솔증가량현저고우무관siRNA조화공백대조조(P<0.01)。결론침묵식관암세포polβ기인표체가증가기대방료적민감성。
Objective To evaluate the effects of silencing DNA polymerase β by small interfering RNA (siRNA) on the radiotherapeutic sensitivity of human esophageal carcinoma cell line EC9706. Methods Various siRNA were transfect-ed into EC9706 cells with lipofectamine technique.Real-time PCR was performed to detect the expression levels of polβ mRNA in different groups,respectively.Three groups of cell were exposed to different doses of radiation (0,2,4,6,8 Gy).Cell survival ratio was detected by means of CCK8 method.And the detection of the apoptosis rate was completed by FCM. Results The expression of polβ mRNA and protein were surpressed obviously in the specific sequence siRNA group (P<0.01).The result of CCK8 showed that cell survival ratio was declining with the increase of radiotherapeutic dose (P<0.05).The cell apoptosis rates of silence polβ group was significantly higher than of not siRNA group and the blank control group (P<0.01),after irradiation,the cell apoptosis rate increase of silence polβ group was significantly higher than of not siRNA group and the blank control group (P<0.01). Conclusion The experiments confirm that si-lencing DNA polymeraseβby siRNA can significantly enhance the radiosensitivity of esophageal carcinoma cell.