当代医学
噹代醫學
당대의학
CHINA CONTEMPORARY MEDICINE
2014年
25期
1-2,3
,共3页
骨质疏松%骨折%骨折愈合%辛伐他汀
骨質疏鬆%骨摺%骨摺愈閤%辛伐他汀
골질소송%골절%골절유합%신벌타정
Osteoporosis%Fracture%Fracture healing%Simvastatin
目的：观察不同剂量辛伐他汀溶液对于骨质疏松大鼠股骨骨折愈合的影响。方法3月龄健康雌性大鼠45只分为5组（n=9），手术组（A）；卵巢切除组（B）；骨质疏松骨折组（C）；骨质疏松性骨折+辛伐他汀1组（D）；骨质疏松性骨折+辛伐他汀2组（E），A组大鼠仅实施假手术不切除双侧卵巢，其余B～D组均要求切除双侧卵巢。C、D、E组在切除双侧卵巢8周后，制左侧股骨中段横行骨折模型，D组在骨折局部注射5 mg/(kg·d)辛伐他汀溶液，E组注射10 mg/(kg·d)辛伐他汀溶液，C组注射不含辛伐他汀的空载体，A、B组在术后8周处死，取出完整左侧股骨，其余3组在骨折造模后4周处死，并取出全部左侧股骨，测定左侧股骨的骨密度、HE染色并光镜下观察。结果（1）切除卵巢8周后，测定B组骨密度显著低于A组（P＜0.05）。（2）骨质疏松骨折3组，C、D、E组的pBMD、mBMD、dBMD、tBMD均显著高于C组（pBMD：F=16.94，P=0.001；mBMD：F=15.57，P=0.001；dBMD：F=24.77，P=0.001；tBMD：F=23.46，P=0.001），D、E 2组中E组各部分骨密度值均高于D组，且有统计学意义（P＜0.05）。（3）形态学观察：HE染色后观察，D、E组较C组而言有较多的板层骨形成，并且D、E组中有较多量的软骨细胞存在，C组中几乎不可见板层骨存在，软骨细胞数量较少，提示D、E组较C组愈合快。结论辛伐他汀溶液皮下注射可以明显加速骨质疏松骨折愈合的进程，而且不同浓度的辛伐他汀溶液对骨质疏松骨折愈合的影响不同。高剂量明显比低剂量效果更为显著。
目的：觀察不同劑量辛伐他汀溶液對于骨質疏鬆大鼠股骨骨摺愈閤的影響。方法3月齡健康雌性大鼠45隻分為5組（n=9），手術組（A）；卵巢切除組（B）；骨質疏鬆骨摺組（C）；骨質疏鬆性骨摺+辛伐他汀1組（D）；骨質疏鬆性骨摺+辛伐他汀2組（E），A組大鼠僅實施假手術不切除雙側卵巢，其餘B～D組均要求切除雙側卵巢。C、D、E組在切除雙側卵巢8週後，製左側股骨中段橫行骨摺模型，D組在骨摺跼部註射5 mg/(kg·d)辛伐他汀溶液，E組註射10 mg/(kg·d)辛伐他汀溶液，C組註射不含辛伐他汀的空載體，A、B組在術後8週處死，取齣完整左側股骨，其餘3組在骨摺造模後4週處死，併取齣全部左側股骨，測定左側股骨的骨密度、HE染色併光鏡下觀察。結果（1）切除卵巢8週後，測定B組骨密度顯著低于A組（P＜0.05）。（2）骨質疏鬆骨摺3組，C、D、E組的pBMD、mBMD、dBMD、tBMD均顯著高于C組（pBMD：F=16.94，P=0.001；mBMD：F=15.57，P=0.001；dBMD：F=24.77，P=0.001；tBMD：F=23.46，P=0.001），D、E 2組中E組各部分骨密度值均高于D組，且有統計學意義（P＜0.05）。（3）形態學觀察：HE染色後觀察，D、E組較C組而言有較多的闆層骨形成，併且D、E組中有較多量的軟骨細胞存在，C組中幾乎不可見闆層骨存在，軟骨細胞數量較少，提示D、E組較C組愈閤快。結論辛伐他汀溶液皮下註射可以明顯加速骨質疏鬆骨摺愈閤的進程，而且不同濃度的辛伐他汀溶液對骨質疏鬆骨摺愈閤的影響不同。高劑量明顯比低劑量效果更為顯著。
목적：관찰불동제량신벌타정용액대우골질소송대서고골골절유합적영향。방법3월령건강자성대서45지분위5조（n=9），수술조（A）；란소절제조（B）；골질소송골절조（C）；골질소송성골절+신벌타정1조（D）；골질소송성골절+신벌타정2조（E），A조대서부실시가수술불절제쌍측란소，기여B～D조균요구절제쌍측란소。C、D、E조재절제쌍측란소8주후，제좌측고골중단횡행골절모형，D조재골절국부주사5 mg/(kg·d)신벌타정용액，E조주사10 mg/(kg·d)신벌타정용액，C조주사불함신벌타정적공재체，A、B조재술후8주처사，취출완정좌측고골，기여3조재골절조모후4주처사，병취출전부좌측고골，측정좌측고골적골밀도、HE염색병광경하관찰。결과（1）절제란소8주후，측정B조골밀도현저저우A조（P＜0.05）。（2）골질소송골절3조，C、D、E조적pBMD、mBMD、dBMD、tBMD균현저고우C조（pBMD：F=16.94，P=0.001；mBMD：F=15.57，P=0.001；dBMD：F=24.77，P=0.001；tBMD：F=23.46，P=0.001），D、E 2조중E조각부분골밀도치균고우D조，차유통계학의의（P＜0.05）。（3）형태학관찰：HE염색후관찰，D、E조교C조이언유교다적판층골형성，병차D、E조중유교다량적연골세포존재，C조중궤호불가견판층골존재，연골세포수량교소，제시D、E조교C조유합쾌。결론신벌타정용액피하주사가이명현가속골질소송골절유합적진정，이차불동농도적신벌타정용액대골질소송골절유합적영향불동。고제량명현비저제량효과경위현저。
Objective To explore the effect of different dose simvastatin solution injected subcutaneously for femur fracture healing of osteoporotic rats.Methods Forty-five 3-mouths old female Wistar rats were randomly divided into 5 groups (n=9). Group A is the sham operation. B-E are the groups with rats which received ovariectomy. The rats in group A、B were sacrificed after 8 weeks under operation. The rats in group C, D and E were further used to establish open fracture models of femur andfixed with intramedullary nails 8 weeks after ovariectomy. C, D and E injected subcutaneously of Simvastatin solutions or vehicle (C with vehicle, D with 5 mg/(kg·d),E with 10mg/(kg·d)). The fractured rats were sacrificed 4 weeks after fracture. Bone mineral density were used to evaluate fracture healing quantitatively,and the femurs were then decalcified for HE staining and subsequent histological observation.Results The BMD of group B is significantly lower than group A after 8 weeks under ovariectomy(P<0.05). BMD of group D and group E are significantly increased than group C. The BMD of group E is significantly higher than group C. The rats in group D and E showed more maturecallus with partially lamellar bone. The observation of histological showed the process of fracture healing of group C with vehicle was delayed.Conclusion The injection of simvastatin solutions can accelerate the process of fracture healing of osteoporotic rats. The highe dose of simvastatin solution can be more efficient than low.
