CAJ | 학술논문

分析了佛手叶挥发油的成分，检测了其生物活性．采用水蒸气蒸馏法提取佛手叶挥发油，气相色谱-质谱联用技术（ GC-MS）检测了佛手叶挥发油的成分，比浊法检测了其抑菌活性，噻唑蓝法检测了其细胞活力．佛手叶挥发油含有40种成分，以d-柠檬烯、γ-松油烯、对伞花烃、α-蒎烯、β-蒎烯为主要成分，其中d-柠檬烯和γ-松油烯的含量分别占50．53％和24．05％．佛手叶挥发油对草绿色链球菌、肺炎克雷伯菌、摩氏摩根菌、大肠杆菌、表皮葡萄球菌和溶血性链球菌等均有抑制作用，其半抑制浓度（ IC50）分别为1．1，2．1，6．8，7．2，13．7和19．3 mg/mL；佛手叶挥发油对Hep3B，HUVEC，Huh7，SP2/0，A549和PANC-28等癌细胞具有抑制作用．
분석료불수협휘발유적성분，검측료기생물활성．채용수증기증류법제취불수협휘발유，기상색보-질보련용기술（ GC-MS）검측료불수협휘발유적성분，비탁법검측료기억균활성，새서람법검측료기세포활력．불수협휘발유함유40충성분，이d-저몽희、γ-송유희、대산화경、α-파희、β-파희위주요성분，기중d-저몽희화γ-송유희적함량분별점50．53％화24．05％．불수협휘발유대초록색련구균、폐염극뢰백균、마씨마근균、대장간균、표피포도구균화용혈성련구균등균유억제작용，기반억제농도（ IC50）분별위1．1，2．1，6．8，7．2，13．7화19．3 mg/mL；불수협휘발유대Hep3B，HUVEC，Huh7，SP2/0，A549화PANC-28등암세포구유억제작용．
It was studied the chemical components and biological activity of volatile oil from fingered citron leaves.Volatile oil was extracted from the fingered citron leaves by steam distillation and its chemical compo-nents were analyzed by GC-MS.The effects of volatile oil on bacteria and cancer cells vitality were assayed by nephelometry method and MTT assay , respectively .40 components were identified , and the main components were d-Limonene and γ-Terpinene , accounting for 50 .53% and 24 .05%, respectively .The results showed that the volatile oil of fingered citron leaves could inhibit the proliferation of Viridans streptococci, Klebsiella pneumonia, Morganella fulton, Escherichia coli, Staphylococcus epidermidis and Hemolytic streptococcus with the IC50 values of 1.1, 2.1, 6.8, 7.2, 13.7 and 19.3 mg/mL, respectively.The volatile oil of fingered cit-ron leaves could also inhibit the viability of Hep 3B, HUVEC, Huh7, SP2/0, A549 and PANC-28.