浙江师范大学学报（自然科学版）
浙江師範大學學報（自然科學版）
절강사범대학학보（자연과학판）
JOURNAL OF ZHEJIANG NORMAL UNIVERSITY(NATURAL SCIENCES)
2014年
3期
323-328
,共6页
籼稻%成恢448%组织培养%激素配比
秈稻%成恢448%組織培養%激素配比
선도%성회448%조직배양%격소배비
indica%Cheng-hui 448%tissue-culture%hormone ratio
对籼稻“成恢448”愈伤组织的诱导和分化过程进行了优化条件的探索．通过正交实验设计了不同的激素配比，结果显示：最佳的愈伤组织诱导条件为MS培养基加入1．0 mg/L 2，4-二氯苯氧乙酸（2，4-D），4．0 mg/L萘乙酸（NAA）和2．0 mg/L 激动素（ KT）；最佳的分化条件为 MS 培养基加入1．0 mg/L NAA，2．0 mg/L 6-苄氨基腺嘌呤（6-BA）和0．5 mg/L KT．
對秈稻“成恢448”愈傷組織的誘導和分化過程進行瞭優化條件的探索．通過正交實驗設計瞭不同的激素配比，結果顯示：最佳的愈傷組織誘導條件為MS培養基加入1．0 mg/L 2，4-二氯苯氧乙痠（2，4-D），4．0 mg/L萘乙痠（NAA）和2．0 mg/L 激動素（ KT）；最佳的分化條件為 MS 培養基加入1．0 mg/L NAA，2．0 mg/L 6-芐氨基腺嘌呤（6-BA）和0．5 mg/L KT．
대선도“성회448”유상조직적유도화분화과정진행료우화조건적탐색．통과정교실험설계료불동적격소배비，결과현시：최가적유상조직유도조건위MS배양기가입1．0 mg/L 2，4-이록분양을산（2，4-D），4．0 mg/L내을산（NAA）화2．0 mg/L 격동소（ KT）；최가적분화조건위 MS 배양기가입1．0 mg/L NAA，2．0 mg/L 6-변안기선표령（6-BA）화0．5 mg/L KT．
Rice indica cultivar Cheng-hui 448, an excellent restorer line in hybrid rice , had resistant to the blast while sensitive to the bacterial blight .Recently, with transgenic technology , a number of resistant genes were applied in rice breeding .However , the tissue-culture systems of indica were still imperfect and signifi-cantly different in varieties .Here, optimization conditions of callus induction and differentiation for the indica variety Cheng-hui 448 was experimented by orthogonal test .According to the results , MS media with 1 .0 mg/L 2,4-D, 4.0 mg/L NAA and 2.0 mg/L KT, MS media with 1.0 mg/L NAA, 2.0 mg/L 6-BA and 0.5 mg/L KT, were the optimization conditions for callus induction and differentiation of Cheng-hui 448, respec-tively .
