CAJ | 학술논문

目的：用戊四氮（PTZ）诱导建立的慢性癫痫（EP）幼鼠模型，研究神经生长因子（NGF）处理后幼鼠海马细胞色素C（Cyt C）的表达，探讨 NGF在 EP发病中的作用。方法50只SD幼鼠随机分为阴性对照组（A组）、阳性对照组（B组）、NGF低剂量组（C组）、NGF中剂量组（D组）、NGF高剂量组（E组）。NGF用药后3 d ，取海马组织用于免疫组织化学及实时荧光定量PCR方法检测Cyt C的表达。结果与A组比较，B组Cyt C阳性细胞数及mRNA 表达量明显增多（P＜0．01）。与B组比较，C～ E组Cyt C阳性细胞数及 mRNA 表达量降低（P＜0．05）。结论 NGF可减少海马Cyt C的表达，促进EP幼鼠体质量增长。
목적：용무사담（PTZ）유도건립적만성전간（EP）유서모형，연구신경생장인자（NGF）처리후유서해마세포색소C（Cyt C）적표체，탐토 NGF재 EP발병중적작용。방법50지SD유서수궤분위음성대조조（A조）、양성대조조（B조）、NGF저제량조（C조）、NGF중제량조（D조）、NGF고제량조（E조）。NGF용약후3 d ，취해마조직용우면역조직화학급실시형광정량PCR방법검측Cyt C적표체。결과여A조비교，B조Cyt C양성세포수급mRNA 표체량명현증다（P＜0．01）。여B조비교，C～ E조Cyt C양성세포수급 mRNA 표체량강저（P＜0．05）。결론 NGF가감소해마Cyt C적표체，촉진EP유서체질량증장。
Objective To explore the effects of nerve growth factor (NGF) on the expression of cytochrome c(Cyt C) in hippocampus of pentylenetetrazol (PTZ)-induced epileptic rats . Methods Fifty SD rats were randomly divided into 5 groups :negative control group (group A ) ,positive control group (group B) ,NGF low-dose group (group C) ,NGF medium-dose group (group D) and NGF high-dose group (group E) . After 3 days of NGF treatment ,the number of Cyt C positive cells and the expression of Cyt C mRNA in hippocampus were detected by immunohistochemisty and real-time PCR . Results The number of Cyt C positive cells and the expression of Cyt C mRNA in hippocampus of group B were significantly higher than group A (P<0 .01) . The number of Cyt C positive cells and the expression of Cyt C mRNA of group C~E were lower than group B (P<0 .05) . Conclusion NGF may play an impor-tant role in decreasing the expression of Cyt C and increasing the weight of epileptic rats .