CAJ | 학술논문

本文为了研究山羊MSTN基因对山羊生长和肌肉发育的作用以及在不同山羊品种间的遗传变异，先期对其5′侧翼区进行了克隆，并利用序列分析方法进行了分析。分析结果表明该克隆序列长度1344bp ，包括5′调控区和5′UTR和部分编码区，含增强子序列、CAAT 盒、TATA盒和起始密码子序列，并存在Oct -1、C/EBP和 Nkx -2.5等多个转录因子的潜在结合位点；同时山羊与绵羊、奶牛、猪和小鼠的同源性分别为98.2％、96.1％、81.2％和59.4％。
본문위료연구산양MSTN기인대산양생장화기육발육적작용이급재불동산양품충간적유전변이，선기대기5′측익구진행료극륭，병이용서렬분석방법진행료분석。분석결과표명해극륭서렬장도1344bp ，포괄5′조공구화5′UTR화부분편마구，함증강자서렬、CAAT 합、TATA합화기시밀마자서렬，병존재Oct -1、C/EBP화 Nkx -2.5등다개전록인자적잠재결합위점；동시산양여면양、내우、저화소서적동원성분별위98.2％、96.1％、81.2％화59.4％。
In order to study the effect of MSTN gene on the grow th and development of muscle and genetic variation among breeds in goats, the 5′-flanking region of MSTN gene was cloned during the preliminary works and analyzed by DNA sequence analysis method in this paper .The results showed that the cloning DNA sequence was composed of 1344bp .According to sequence analysis ,it contains 5′-promoter, 5′-UTR and partial cds .Furthermore some regulatory elements ,such as enhancer sequence, CCAAT box , TATA boxes ,start codon sequence and some potential transcription factor binding sites ,for example Oct-1、C/EBP and Nkx -2.5, were found in the cloning sequence. The homologies of the sequence of goat with sheep, dairy cattle, porcine, and mice were 98.2%, 96. 1%, 81. 2% and 59.4%, respectively.