CAJ | 학술논문

目的：研制一种新型的碳磷灰石骨修复材料，初步观察其成骨作用。方法：以鸡蛋壳为原料，采用微波法制备不同加热条件下的骨修复材料，利用扫描电镜（SEM）、X射线衍射仪（XRD）、傅立叶红外（FT-IR）和能谱分析（EDXA）手段，表征微波处理前后材料的理化性能。进一步选用新西兰兔制备直径5mm的颅骨全层缺损区，以未经微波处理的鸡蛋壳粉及Bio-Oss作为对照，充填经微波处理后的新型骨修复材料，8周后观察新生骨的生成情况。结果：SEM, XRD, FT-IR和EDXA分析证实：随加热时间延长，材料的微观形态由针状向球状转变，碳磷灰石的特征性波峰也不断增强；研制的新材料含有与人体骨相似的碳、磷和钙成分。动物实验的结果表明，经微波处理的碳磷灰石鸡蛋壳粉充填的缺损区，比未处理组和空白对照组的缺损区有更多的矿化新生骨和较少的未矿化新生骨，接近Bio-Oss的效果。结论：微波加热法能有效地将天然碳酸钙转化为碳磷灰石，该材料有望作为一种潜在的新型骨修复材料。
목적：연제일충신형적탄린회석골수복재료，초보관찰기성골작용。방법：이계단각위원료，채용미파법제비불동가열조건하적골수복재료，이용소묘전경（SEM）、X사선연사의（XRD）、부립협홍외（FT-IR）화능보분석（EDXA）수단，표정미파처리전후재료적이화성능。진일보선용신서란토제비직경5mm적로골전층결손구，이미경미파처리적계단각분급Bio-Oss작위대조，충전경미파처리후적신형골수복재료，8주후관찰신생골적생성정황。결과：SEM, XRD, FT-IR화EDXA분석증실：수가열시간연장，재료적미관형태유침상향구상전변，탄린회석적특정성파봉야불단증강；연제적신재료함유여인체골상사적탄、린화개성분。동물실험적결과표명，경미파처리적탄린회석계단각분충전적결손구，비미처리조화공백대조조적결손구유경다적광화신생골화교소적미광화신생골，접근Bio-Oss적효과。결론：미파가열법능유효지장천연탄산개전화위탄린회석，해재료유망작위일충잠재적신형골수복재료。
Objective To develop a new bone substitute derived from avian eggshell (ES) using micro-wave processing and observe its abilities of bone formation. Methods Calcium carbonate ES powder was pre-pared by microwave method with different heating time. The physicochemical characteristics of the new materials with or without microwave heating were analyzed by SEM、EDXA、XRD、FT-IR . A pilot animal study was conducted by creating standard defects of 5 mm diameter on parietal bone of New Zealand rabbits. The defects were filled with 60 min microwave heated ES powder whereas using microwave untreated ES powder and Bio-Oss in comparison. After 8 weeks new bone formation of defects were observed. Results SEM, XRD, FT-IR and EDXA analysis showed the micromorphology of the new materials turned from needle-like to ball-like structures as the microwave heating time prolonged. The characteristic peak of calcium carbonate was also increased at the same time. Carbon, phosphorus and calcium were confirmed in the new material as well. Animal study showed 5 mm defects on parietal bone filled with calcium carbonate ES powder had more calcified bone formation and less uncalcified new bone. Conclusions The study demonstrated the efficient use of microwave in converting ES powder from natural CaCO3 into calcium carbonate. The new material might be a potential bone substitute.