CAJ | 학술논문

采用胶体金标记鼠抗人IgMμ链抗体，肺炎支原体P1抗原作包被蛋白，检测肺炎支原体特异性抗体IgM．经对免疫金制备条件进行优化，制备了胶体金免疫层析检测试纸条．将该试纸条与市售试剂盒作对比检测，总符合率为99．17％，表明本方法灵敏度较高、特异性较强，价格低，操作简便快捷，更适合MP感染患者早期临床筛查．
채용효체금표기서항인IgMμ련항체，폐염지원체P1항원작포피단백，검측폐염지원체특이성항체IgM．경대면역금제비조건진행우화，제비료효체금면역층석검측시지조．장해시지조여시수시제합작대비검측，총부합솔위99．17％，표명본방법령민도교고、특이성교강，개격저，조작간편쾌첩，경괄합MP감염환자조기림상사사．
Using colloidal gold-labeled mouse anti-human IgMμchain antibody,Mycoplasma pneumoniae P1 protein antigen for coating,immuno-gold preparation conditions were optimized to prepare colloidal gold immunochromatographic test strip.The test strip kit was contrasted with that in market,total coincidence rate was 99.17%.The results showed that the test strip had high sensitivity,specificity,low price.It was more suitable for MP infection in early clinical screening,and was easy and quick to operate.