中西医结合肝病杂志
中西醫結閤肝病雜誌
중서의결합간병잡지
CHINESE JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE ON LIVER DISEASES
2014年
4期
239-240,242
,共3页
暴发性肝衰竭%肝再生%增殖细胞核抗原%丹参注射液/药理作用%大鼠
暴髮性肝衰竭%肝再生%增殖細胞覈抗原%丹參註射液/藥理作用%大鼠
폭발성간쇠갈%간재생%증식세포핵항원%단삼주사액/약리작용%대서
actue liver failure%liver regeneration%proliferation cell nucier antigen%Radix Salviae Miltiorrhizae/pharma-cological effect%rat
目的：探讨丹参对暴发性肝衰竭（ FHF）大鼠肝损伤及肝再生的影响。方法： Wistar大鼠随机分为4组：正常对照组、 FHF组、丹参组、促肝细胞生长素（ PHGF）组。 FHF、丹参组和PHGF组动物模型采用TAA皮下注射，剂量按每kg体重600mg，2次，每次间隔24h，复制FHF动物模型。丹参组和PHGF组动物除皮下注射TAA外，于实验前3天至实验结束分别于皮下注射丹参注射液1ml/100g和PHGF1ml/100g，正常对照组和FHF组大鼠同时皮下注射生理盐水1ml/100g。 FHF组、丹参组和PHGF组，于第2次注射TAA后24h，随机取大鼠各8只，腹主动脉取血测肝功能。迅速取肝组织，用10％甲醛液固定，制成石蜡切片，检测肝细胞有丝分裂指数（ MI）和增殖细胞核抗原（PCNA）。结果：丹参具有降低FHF大鼠ALT、 AST及TBil，显著提高肝细胞MI和PCNA的作用（P＜0.05， P＜0.01）。结论：丹参具有改善FHF大鼠肝功能和促进肝细胞增殖及再生的作用。
目的：探討丹參對暴髮性肝衰竭（ FHF）大鼠肝損傷及肝再生的影響。方法： Wistar大鼠隨機分為4組：正常對照組、 FHF組、丹參組、促肝細胞生長素（ PHGF）組。 FHF、丹參組和PHGF組動物模型採用TAA皮下註射，劑量按每kg體重600mg，2次，每次間隔24h，複製FHF動物模型。丹參組和PHGF組動物除皮下註射TAA外，于實驗前3天至實驗結束分彆于皮下註射丹參註射液1ml/100g和PHGF1ml/100g，正常對照組和FHF組大鼠同時皮下註射生理鹽水1ml/100g。 FHF組、丹參組和PHGF組，于第2次註射TAA後24h，隨機取大鼠各8隻，腹主動脈取血測肝功能。迅速取肝組織，用10％甲醛液固定，製成石蠟切片，檢測肝細胞有絲分裂指數（ MI）和增殖細胞覈抗原（PCNA）。結果：丹參具有降低FHF大鼠ALT、 AST及TBil，顯著提高肝細胞MI和PCNA的作用（P＜0.05， P＜0.01）。結論：丹參具有改善FHF大鼠肝功能和促進肝細胞增殖及再生的作用。
목적：탐토단삼대폭발성간쇠갈（ FHF）대서간손상급간재생적영향。방법： Wistar대서수궤분위4조：정상대조조、 FHF조、단삼조、촉간세포생장소（ PHGF）조。 FHF、단삼조화PHGF조동물모형채용TAA피하주사，제량안매kg체중600mg，2차，매차간격24h，복제FHF동물모형。단삼조화PHGF조동물제피하주사TAA외，우실험전3천지실험결속분별우피하주사단삼주사액1ml/100g화PHGF1ml/100g，정상대조조화FHF조대서동시피하주사생리염수1ml/100g。 FHF조、단삼조화PHGF조，우제2차주사TAA후24h，수궤취대서각8지，복주동맥취혈측간공능。신속취간조직，용10％갑철액고정，제성석사절편，검측간세포유사분렬지수（ MI）화증식세포핵항원（PCNA）。결과：단삼구유강저FHF대서ALT、 AST급TBil，현저제고간세포MI화PCNA적작용（P＜0.05， P＜0.01）。결론：단삼구유개선FHF대서간공능화촉진간세포증식급재생적작용。
Objective: To study the effect of Radix Salviae Miltiorrhizae on liver damage and liver regeneration in thioacet-amide-induced acute hepatic failure in rats.Methods: The rats were randomly divided into normal group , model group , Radix Salviae Miltiorrhizae group and lyophilized bovine transfer factor control group.Medications were given 1ml/100g respectively 3 days before hypodermically injected with thioacetamide ( TAA ) once a day.The rats were hypodermically injected with TAA 600mg· kg-1 twice at same time for two days to induce the model of acute hepatic failure rat except the normal group injected with the 0.9%salt solution hypodermically.Forty-eight hours later , the blood were collected to measure ALT , AST, TBil, and at the same time, the parameters of liver regeneration were observed as following: liver mitotic index and proliferation cell nucler antigen.Results: Radix Salviae Miltiorrhizae could obviously decrease ALT , AST, TBil, improved liver mitotic index and pro-liferation cell nucier antigen of rats with acute hepatic failure.Comparing with model group and control group , there were signifi-cant difference (P<0.05 or P<0.01) .Conclusion: Radix Salviae Miltiorrhizae has esistance against the rat with acute he-patic failure , improve liver regeneration.
