CAJ | 학술논문

目的：研究年龄与性别对异硫氰酸萘酯（ ANIT ）中毒所致小鼠急性肝损伤的影响。方法：采用一次性ANIT 75mg/Kg经口灌胃染毒致5、10、20周龄雌雄性小鼠急性肝损伤，通过肝功能（ ALT， AST、 DBil）检测和肝组织HE染色病理学检查，观察不同性别和年龄小鼠急性肝损伤变化特征。结果： ANIT 75mg/Kg经口灌胃染毒可致5周龄小鼠血清ALT、 AST和DBil明显上升，肝组织学呈明显淤胆改变。随着年龄增加， ANIT中毒所致小鼠上述改变更加明显。雄性小鼠明显重于雌性小鼠，雌性小鼠怀孕后肝损伤胆红素上升， ANIT 100mg/Kg经口灌胃可致雄性老年小鼠出现死亡。结论： ANIT中毒所致小鼠急性肝损伤与年龄和性别密切相关，毒性以雄性老年小鼠更加明显，只适合用于制作原发性硬化性胆管炎（ PSC）动物模型。
목적：연구년령여성별대이류청산내지（ ANIT ）중독소치소서급성간손상적영향。방법：채용일차성ANIT 75mg/Kg경구관위염독치5、10、20주령자웅성소서급성간손상，통과간공능（ ALT， AST、 DBil）검측화간조직HE염색병이학검사，관찰불동성별화년령소서급성간손상변화특정。결과： ANIT 75mg/Kg경구관위염독가치5주령소서혈청ALT、 AST화DBil명현상승，간조직학정명현어담개변。수착년령증가， ANIT중독소치소서상술개변경가명현。웅성소서명현중우자성소서，자성소서부잉후간손상담홍소상승， ANIT 100mg/Kg경구관위가치웅성노년소서출현사망。결론： ANIT중독소치소서급성간손상여년령화성별밀절상관，독성이웅성노년소서경가명현，지괄합용우제작원발성경화성담관염（ PSC）동물모형。
Objective: To study the effects of age and gender on α-naphthylisothiocyanate ( ANIT) -induced acute toxic liver injury in mice.Methods: Male and female mice of 5, 10, 20 weeks olds were gavaged orally with ANIT 75mg/Kg once a time to induce acute liver injury , serum biochemical parameters of liver function [ including alanine aminotransferase ( ALT) and aspartate aminotransferase (AST), direct bilirubin (DBil) ] and HE staining of liver tissue were detected to observe the charac-teristic changes of acute liver injury with different age and gender.Results: Oral gavage of ANIT 75mg/Kg caused significant in-crease of serum ALT, AST and DBil in mice of 5 weeks old, apparent bile stasis change of liver tissue.With the increase of age , the above changes became more evident.The above changes were more severe in male mice than in female mice , meanwhile after pregnancy in female mice liver injury became more severe , and more, ANIT 100mg/Kg led to deaths in male aged mice.Conclusion: The acute liver injury in mice induced by ANIT is closely related with age and gender , the injury is more pro-nounced in male aged mice , which is only suitable to produce animal model of primary sclerosing cholangitis.