质量技术监督研究
質量技術鑑督研究
질량기술감독연구
QUALITY AND TECHNICAL SUPERVISION RESEARCH
2014年
4期
2-5
,共4页
头发%碱性染料%HPLC
頭髮%堿性染料%HPLC
두발%감성염료%HPLC
Hair%Basic dyes%HPLC
文中建立了HPLC-DAD同时测定头发中碱性橙31等9种碱性染料的方法。方法以甲醇为提取溶剂，采用XB-C18柱，以乙腈-0.02mol/L乙酸铵(含0.05%甲酸)为流动相进行梯度洗脱，二极管阵列检测器检测。试验的平均回收率为93.9%～102.5%，相对标准偏差为1.2%～3.5%，最低检测浓度为0.15～0.60 mg/kg。结果表明该方法前处理简单、快速，适用于测定头发中碱性橙31等9种碱性染料组分的含量。
文中建立瞭HPLC-DAD同時測定頭髮中堿性橙31等9種堿性染料的方法。方法以甲醇為提取溶劑，採用XB-C18柱，以乙腈-0.02mol/L乙痠銨(含0.05%甲痠)為流動相進行梯度洗脫，二極管陣列檢測器檢測。試驗的平均迴收率為93.9%～102.5%，相對標準偏差為1.2%～3.5%，最低檢測濃度為0.15～0.60 mg/kg。結果錶明該方法前處理簡單、快速，適用于測定頭髮中堿性橙31等9種堿性染料組分的含量。
문중건립료HPLC-DAD동시측정두발중감성등31등9충감성염료적방법。방법이갑순위제취용제，채용XB-C18주，이을정-0.02mol/L을산안(함0.05%갑산)위류동상진행제도세탈，이겁관진렬검측기검측。시험적평균회수솔위93.9%～102.5%，상대표준편차위1.2%～3.5%，최저검측농도위0.15～0.60 mg/kg。결과표명해방법전처리간단、쾌속，괄용우측정두발중감성등31등9충감성염료조분적함량。
A HPLC-DAD method of simultaneous determination of 9 basic dyes in hair was developed. Hair samples were subject to extraction using methanol, the exact was performed on a XB-C18 column with a diode array detector of acetonitrile-0.02mol/L ammonium acetate(contain 0.05%(v/v) formic acid). Recovery rates with 9 basic dyes were in the range of 93.9%-102.5%,with the RSD of 1.2%-3.5%.The minimum detectable concentration was 0.15-0.60 mg/kg. The results show that the method is simple, rapid, and suitable for the determination of 9 basic dyes’ content.
