CAJ | 학술논문

目的：研究颅脑创伤后大鼠脑组织中Apelin表达的变化规律。方法96只雄性SD大鼠随机分为假手术组(A组)、轻型颅脑创伤组（B组）和重型颅脑创伤组（C组），每组又分6小时、24小时、72小时、168小时4亚组，RT-PCR法观测 Apelin的基因表达，免疫组化法观测Apelin的表达。结果 B组和C组的Apelin表达高于A组，并在24小时达到高峰，而且在C组的升高程度大于 B组。结论颅脑损伤后脑组织中Apelin的表达增加，并且与损伤严重程度相关。
목적：연구로뇌창상후대서뇌조직중Apelin표체적변화규률。방법96지웅성SD대서수궤분위가수술조(A조)、경형로뇌창상조（B조）화중형로뇌창상조（C조），매조우분6소시、24소시、72소시、168소시4아조，RT-PCR법관측 Apelin적기인표체，면역조화법관측Apelin적표체。결과 B조화C조적Apelin표체고우A조，병재24소시체도고봉，이차재C조적승고정도대우 B조。결론로뇌손상후뇌조직중Apelin적표체증가，병차여손상엄중정도상관。
Objective To study the changes of Apelin in the rat after traumatic brain injury. Methods 96 male SD rats were randomly di-vided into 3 groups, namely the sham operation group (group A), the mild traumatic brain injury group (group B) and the severe traumatic brain injury group (group C). Each group was further divided into four sub-groups according to the time interval between injuries and scarify of the rats. The ex-pression of Apelin in the brain tissue was detected by immunohistochemical technique and RT-PCR. Results The expression of Apelin in the group B and C were both significantly higher than that in the group A (P<0.05), It reached a peak 24h after injury. Furthermore it had higher level in group C than that in group B (P<0.05). Conclusion The results suggested that Apelin was up-regulated obviously in the brain after traumatic brain injury, and it was assosiated with injury severity.